Format

Send to

Choose Destination
Mamm Genome. 2016 Dec;27(11-12):538-555. Epub 2016 Aug 18.

Mouse models of Down syndrome: gene content and consequences.

Author information

1
Department of Pediatrics, Linda Crnic Institute for Down Syndrome, University of Colorado Denver School of Medicine, Aurora, CO, USA.
2
Department of Pediatrics, Linda Crnic Institute for Down Syndrome, University of Colorado Denver School of Medicine, Aurora, CO, USA. Katheleen.gardiner@ucdenver.edu.
3
Department of Biochemistry and Molecular Biology, University of Colorado Denver School of Medicine, Aurora, CO, USA. Katheleen.gardiner@ucdenver.edu.
4
Human Medical Genetics and Genomics, and Neuroscience Programs, University of Colorado Denver School of Medicine, 12700 E 19th Avenue, Mail Stop 8608, Aurora, CO, 80045, USA. Katheleen.gardiner@ucdenver.edu.

Abstract

Down syndrome (DS), trisomy of human chromosome 21 (Hsa21), is challenging to model in mice. Not only is it a contiguous gene syndrome spanning 35 Mb of the long arm of Hsa21, but orthologs of Hsa21 genes map to segments of three mouse chromosomes, Mmu16, Mmu17, and Mmu10. The Ts65Dn was the first viable segmental trisomy mouse model for DS; it is a partial trisomy currently popular in preclinical evaluations of drugs for cognition in DS. Limitations of the Ts65Dn are as follows: (i) it is trisomic for 125 human protein-coding orthologs, but only 90 of these are Hsa21 orthologs and (ii) it lacks trisomy for ~75 Hsa21 orthologs. In recent years, several additional mouse models of DS have been generated, each trisomic for a different subset of Hsa21 genes or their orthologs. To best exploit these models and interpret the results obtained with them, prior to proposing clinical trials, an understanding of their trisomic gene content, relative to full trisomy 21, is necessary. Here we first review the functional information on Hsa21 protein-coding genes and the more recent annotation of a large number of functional RNA genes. We then discuss the conservation and genomic distribution of Hsa21 orthologs in the mouse genome and the distribution of mouse-specific genes. Lastly, we consider the strengths and weaknesses of mouse models of DS based on the number and nature of the Hsa21 orthologs that are, and are not, trisomic in each, and discuss their validity for use in preclinical evaluations of drug responses.

PMID:
27538963
PMCID:
PMC5471624
DOI:
10.1007/s00335-016-9661-8
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Springer Icon for PubMed Central
Loading ...
Support Center