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Elife. 2016 Aug 18;5. pii: e18167. doi: 10.7554/eLife.18167.

Human pyramidal to interneuron synapses are mediated by multi-vesicular release and multiple docked vesicles.

Author information

1
MTA-SZTE Research Group for Cortical Microcircuits, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Szeged, Hungary.
2
Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary.
3
Department of Neurosurgery, University of Szeged, Szeged, Hungary.

Abstract

Classic theories link cognitive abilities to synaptic properties and human-specific biophysical features of synapses might contribute to the unparalleled performance of the human cerebral cortex. Paired recordings and multiple probability fluctuation analysis revealed similar quantal sizes, but 4-times more functional release sites in human pyramidal cell to fast-spiking interneuron connections compared to rats. These connections were mediated on average by three synaptic contacts in both species. Each presynaptic active zone (AZ) contains 6.2 release sites in human, but only 1.6 in rats. Electron microscopy (EM) and EM tomography showed that an AZ harbors 4 docked vesicles in human, but only a single one in rats. Consequently, a Katz's functional release site occupies ~0.012 μm(2) in the human presynaptic AZ and ~0.025 μm(2) in the rat. Our results reveal a robust difference in the biophysical properties of a well-defined synaptic connection of the cortical microcircuit of human and rodents.

KEYWORDS:

EPSC; cerebral cortex; human; interneuron; neuroscience; quantal analysis; rat; release site

PMID:
27536876
PMCID:
PMC4999310
DOI:
10.7554/eLife.18167
[Indexed for MEDLINE]
Free PMC Article

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