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J Clin Microbiol. 2016 Nov;54(11):2832-2836. Epub 2016 Aug 17.

Rapid Identification of OXA-48 and OXA-163 Subfamilies in Carbapenem-Resistant Gram-Negative Bacilli with a Novel Immunochromatographic Lateral Flow Assay.

Author information

1
Servicio Antimicrobianos, Laboratorio Nacional y Regional de Referencia en Antimicrobianos, Instituto Nacional de Enfermedades Infecciosas (INEI), ANLIS Dr. Carlos G. Malbrán, Ministerio de Salud de la Nación, Ciudad Autónoma de Buenos Aires, Argentina fpasteran@gmail.com.
2
Coris BioConcept, Gembloux, Belgium.
3
Servicio Antimicrobianos, Laboratorio Nacional y Regional de Referencia en Antimicrobianos, Instituto Nacional de Enfermedades Infecciosas (INEI), ANLIS Dr. Carlos G. Malbrán, Ministerio de Salud de la Nación, Ciudad Autónoma de Buenos Aires, Argentina.
4
Laboratorio de Resistencia Bacteriana, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
5
National Reference Laboratory for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant-Godinne, UCL Namur, Yvoir, Belgium.

Abstract

We assessed a novel immunochromatographic lateral flow assay for direct identification of OXA-48-like carbapenemases and accurate differentiation of allele variants with distinct substrate profiles (OXA-48 or OXA-163 subfamilies). The assay allowed rapid (less than 4 min) and reliable direct confirmation of OXA-163- and/or OXA-48-like enzymes (with 100% sensitivity and 100% specificity) from cultured colonies that were recovered from both solid medium and spiked blood culture bottles.

PMID:
27535687
PMCID:
PMC5078564
DOI:
10.1128/JCM.01175-16
[Indexed for MEDLINE]
Free PMC Article

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