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Biochim Biophys Acta. 2016 Nov;1861(11):1746-1755. doi: 10.1016/j.bbalip.2016.08.005. Epub 2016 Aug 12.

Omega-3 fatty acid supplementation influences the whole blood transcriptome in women with obesity, associated with pro-resolving lipid mediator production.

Author information

1
Department of Clinical Biochemistry, Collegium Medicum, Jagiellonian University, Krakow, Poland. Electronic address: apolus@cm-uj.krakow.pl.
2
Department of Clinical Biochemistry, Collegium Medicum, Jagiellonian University, Krakow, Poland.
3
Department of Molecular Biology and Clinical Genetics Laboratory, Collegium Medicum, Jagiellonian University, Krakow, Poland.
4
Department of Metabolic Disorders, Collegium Medicum, Jagiellonian University, Krakow, Poland.
5
Human Development and Health Academic Unit, Faculty of Medicine, University of Southampton, Southampton, United Kingdom.
6
Human Development and Health Academic Unit, Faculty of Medicine, University of Southampton, Southampton, United Kingdom; NIHR Southampton Biomedical Research Centre, University Hospital Southampton NHS Foundation Trust, University of Southampton, Southampton, United Kingdom.

Abstract

The n-3 polyunsaturated fatty acids (PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) may reduce low-grade inflammation associated with obesity. The relationship between therapeutic response to n-3 PUFAs and modification of the transcriptome in obesity or metabolic syndrome remains to be explored. Blood samples were obtained from women with obesity before and after three-months supplementation with a moderate dose of n-3 PUFAs (1.8g EPA+DHA per day) or from controls. n-3 PUFAs (GC) and plasma concentrations of lipoxins, resolvins, protectin X (GC-MS/MS) and inflammatory markers (ELISA) were measured. Whole blood transcriptome was assayed using microarray. Women supplemented with n-3 PUFAs for 3months had significantly higher levels of EPA and DHA in plasma phosphatidylcholine. n-3 PUFA supplementation, in contrast to placebo, significantly decreased the concentrations of several inflammatory markers (SELE, MCP-1, sVCAM-1, sPECAM-1, and hsCRP), fasting triglycerides and insulin and increased the concentrations of pro-resolving DHA derivatives in plasma. The microarray data demonstrated effects of n-3 PUFAs on PPAR-α, NRF2 and NF-κB target genes. N-3 PUFAs increased DHA-derived pro-resolving mediators in women with obesity. Elevated resolvins and up-regulation of the resolvin receptor occurred in parallel with activation of PPAR-α target genes related to lipid metabolism and of NRF2 up-regulated antioxidant enzymes.

KEYWORDS:

DHA and EPA supplementation; Human obesity; Inflammation; Microarray; Pro-resolving mediators

PMID:
27531277
DOI:
10.1016/j.bbalip.2016.08.005
[Indexed for MEDLINE]

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