A novel inositol-phospholipid-specific phospholipase C. Rapid purification and characterization

Eur J Biochem. 1989 Jul 1;182(3):673-7. doi: 10.1111/j.1432-1033.1989.tb14878.x.

Abstract

A novel bovine brain inositol-phospholipid-specific phospholipase C has been identified on the basis of chromatographic behaviour and purified to apparent homogeneity by a rapid three-step procedure. The purified enzyme has a molecular mass of 85 kDa on SDS/polyacrylamide gel electrophoresis and a specific activity of 24 mumol.min-1.mg-1. The enzyme is dependent on Ca2+ and shows a marked preference for inositol phospholipid substrates. The unique nature of this polypeptide was confirmed through partial protein sequence analysis.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Brain / enzymology
  • Brain / metabolism
  • Cattle
  • Chromatography / methods
  • Electrophoresis, Polyacrylamide Gel
  • Hydrolysis
  • Inositol Phosphates / metabolism*
  • Molecular Sequence Data
  • Peptide Fragments / isolation & purification
  • Peptides / isolation & purification
  • Phospholipids / metabolism*
  • Sugar Phosphates / metabolism*
  • Type C Phospholipases / genetics
  • Type C Phospholipases / isolation & purification*
  • Type C Phospholipases / metabolism

Substances

  • Inositol Phosphates
  • Peptide Fragments
  • Peptides
  • Phospholipids
  • Sugar Phosphates
  • Type C Phospholipases