Abstract
A novel bovine brain inositol-phospholipid-specific phospholipase C has been identified on the basis of chromatographic behaviour and purified to apparent homogeneity by a rapid three-step procedure. The purified enzyme has a molecular mass of 85 kDa on SDS/polyacrylamide gel electrophoresis and a specific activity of 24 mumol.min-1.mg-1. The enzyme is dependent on Ca2+ and shows a marked preference for inositol phospholipid substrates. The unique nature of this polypeptide was confirmed through partial protein sequence analysis.
MeSH terms
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Amino Acid Sequence
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Animals
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Brain / enzymology
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Brain / metabolism
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Cattle
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Chromatography / methods
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Electrophoresis, Polyacrylamide Gel
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Hydrolysis
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Inositol Phosphates / metabolism*
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Molecular Sequence Data
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Peptide Fragments / isolation & purification
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Peptides / isolation & purification
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Phospholipids / metabolism*
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Sugar Phosphates / metabolism*
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Type C Phospholipases / genetics
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Type C Phospholipases / isolation & purification*
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Type C Phospholipases / metabolism
Substances
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Inositol Phosphates
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Peptide Fragments
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Peptides
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Phospholipids
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Sugar Phosphates
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Type C Phospholipases