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J Dermatol Sci. 2016 Nov;84(2):169-177. doi: 10.1016/j.jdermsci.2016.08.004. Epub 2016 Aug 4.

Lemon balm extract (Melissa officinalis, L.) promotes melanogenesis and prevents UVB-induced oxidative stress and DNA damage in a skin cell model.

Author information

1
Institute of Molecular and Cell Biology (IBMC), Miguel Hernández University (UMH), Avenida de la Universidad s/n, E-03202 Elche, Alicante, Spain.
2
Institute of Molecular and Cell Biology (IBMC), Miguel Hernández University (UMH), Avenida de la Universidad s/n, E-03202 Elche, Alicante, Spain; INVITROTECNIA S.L., Santiago Grisolía 2, 28760 Tres Cantos, Madrid, Spain.
3
Nutrafur S.A., Camino Viejo de Pliego, km.2, 30820 Alcantarilla, Murcia, Spain; Department of Food Technology and Nutrition, Universidad Católica San Antonio, Murcia, Spain.
4
Institute of Molecular and Cell Biology (IBMC), Miguel Hernández University (UMH), Avenida de la Universidad s/n, E-03202 Elche, Alicante, Spain; CIBER (CB12/03/30038, Fisiopatología de la Obesidad y la Nutrición, CIBERobn, Instituto de Salud Carlos III), Spain. Electronic address: vmicol@umh.es.

Abstract

BACKGROUND:

Solar ultraviolet (UV) radiation is one of the main causes of a variety of cutaneous disorders, including photoaging and skin cancer. Its UVB component (280-315nm) leads to oxidative stress and causes inflammation, DNA damage, p53 induction and lipid and protein oxidation. Recently, an increase in the use of plant polyphenols with antioxidant and anti-inflammatory properties has emerged to protect human skin against the deleterious effects of sunlight.

OBJECTIVE:

This study evaluates the protective effects of lemon balm extract (LBE) (Melissa Officinalis, L) and its main phenolic compound rosmarinic acid (RA) against UVB-induced damage in human keratinocytes.

METHODS:

The LBE composition was determined by HPLC analysis coupled to photodiode array detector and ion trap mass spectrometry with electrospray ionization (HPLC-DAD-ESI-IT-MS/MS). Cell survival, ROS generation and DNA damage were determined upon UVB irradiation in the presence of LBE. The melanogenic capacity of LBE was also determined.

RESULTS:

RA and salvianolic acid derivatives were the major compounds, but caffeic acid and luteolin glucuronide were also found in LBE. LBE and RA significantly increased the survival of human keratinocytes upon UVB radiation, but LBE showed a stronger effect. LBE significantly decreased UVB-induced intracellular ROS production. Moreover, LBE reduced UV-induced DNA damage and the DNA damage response (DDR), which were measured as DNA strand breaks in the comet assay and histone H2AX activation, respectively. Finally, LBE promoted melanogenesis in the cell model.

CONCLUSIONS:

These results suggest that LBE may be considered as a candidate for the development of oral/topical photoprotective ingredients against UVB-induced skin damage.

KEYWORDS:

DNA damage; Keratinocytes; Lemon balm extract; Reactive oxygen species (ROS); Rosmarinic acid; UV radiation

PMID:
27528586
DOI:
10.1016/j.jdermsci.2016.08.004
[Indexed for MEDLINE]

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