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Peptides. 2016 Oct;84:7-16. doi: 10.1016/j.peptides.2016.07.005. Epub 2016 Aug 12.

Two novel antimicrobial defensins from rice identified by gene coexpression network analyses.

Author information

1
Biotechnology Program, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: supaluk.tan@student.chula.ac.th.
2
Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand. Electronic address: onanong@swu.ac.th.
3
Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: wanich_oil@hotmail.com.
4
Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: a.meeprasert@gmail.com.
5
Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: thanyada.r@chula.ac.th.
6
Department of Chemistry, Faculty of Science, Mahasarakham University, Mahasarakham 44150, Thailand. Electronic address: rakrudee.s@msu.ac.th.
7
National Center for Genetic Engineering and Biotechnology (BIOTEC), Klong Luang, Pathumthani, 12120, Thailand. Electronic address: sittiruk@biotec.or.th.
8
Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Omics Sciences and Bioinformatics Center, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: supaart.s@chula.ac.th.

Abstract

Defensins form an antimicrobial peptides (AMP) family, and have been widely studied in various plants because of their considerable inhibitory functions. However, their roles in rice (Oryza sativa L.) have not been characterized, even though rice is one of the most important staple crops that is susceptible to damaging infections. Additionally, a previous study identified 598 rice genes encoding cysteine-rich peptides, suggesting there are several uncharacterized AMPs in rice. We performed in silico gene expression and coexpression network analyses of all genes encoding defensin and defensin-like peptides, and determined that OsDEF7 and OsDEF8 are coexpressed with pathogen-responsive genes. Recombinant OsDEF7 and OsDEF8 could form homodimers. They inhibited the growth of the bacteria Xanthomonas oryzae pv. oryzae, X. oryzae pv. oryzicola, and Erwinia carotovora subsp. atroseptica with minimum inhibitory concentration (MIC) ranging from 0.6 to 63μg/mL. However, these OsDEFs are weakly active against the phytopathogenic fungi Helminthosporium oryzae and Fusarium oxysporum f.sp. cubense. This study describes a useful method for identifying potential plant AMPs with biological activities.

KEYWORDS:

Coexpression network analysis; Defensin; Recombinant peptide production; Rice

PMID:
27527801
DOI:
10.1016/j.peptides.2016.07.005
[Indexed for MEDLINE]

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