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Vet Microbiol. 2016 Aug 30;192:175-180. doi: 10.1016/j.vetmic.2016.07.014. Epub 2016 Jul 25.

Maternally-derived antibodies (MDAs) impair piglets' humoral and cellular immune responses to vaccination against porcine reproductive and respiratory syndrome (PRRS).

Author information

1
Agence Nationale de Sécurité Sanitaire (ANSES), Unité Epidémiologie et Bien-Etre du Porc, B.P. 53, 22440 Ploufragan, France; Université Bretagne Loire, France. Electronic address: christelle.fablet@anses.fr.
2
Agence Nationale de Sécurité Sanitaire (ANSES), Unité Virologie Immunologie Porcines, B.P. 53, 22440 Ploufragan, France; Union des Groupements de Producteurs de Viande de Bretagne (UGPVB), 104 rue Eugène Pottier, CS 26553, 35065 Rennes, France; Université Bretagne Loire, France.
3
Agence Nationale de Sécurité Sanitaire (ANSES), Unité Epidémiologie et Bien-Etre du Porc, B.P. 53, 22440 Ploufragan, France; Université Bretagne Loire, France.
4
Agence Nationale de Sécurité Sanitaire (ANSES), Unité Virologie Immunologie Porcines, B.P. 53, 22440 Ploufragan, France; Université Bretagne Loire, France.
5
Porc. Spective, Groupe Vétérinaire Chêne Vert Conseil, 56920 Noyal-Pontivy, France.

Abstract

The influence of maternally-derived antibodies (MDAs) on the post-vaccination humoral and cellular immune responses in piglets vaccinated against PRRS was studied. The piglets came from a vaccinated breeding herd. Thirty piglets with a low (A-) or high level (A+) of PRRSV-neutralizing MDAs were vaccinated (V+) with a modified live vaccine at 3 weeks of age. Blood samples were collected before vaccination and then at 2, 4, 8 and 14 weeks post-vaccination (WPV). The samples were analysed to detect the vaccine viraemia (RT-PCR) and quantify the post-vaccination humoral (ELISA and virus neutralisation test) and cellular (ELISPOT IFNγ) immune responses. PRRSV vaccine strain was detected in 60%, 64%, 36% and 0% of A-V+ piglets 2, 4, 8 and 14 WPV respectively. No virus was detected in A+V+ piglets during the first four WPV but 32% and 6% of A+V+ piglets were PCR-positive at 8 and 14 WPV. Eighty-five percent of A-V+ piglets and 0% of A+V+ piglets seroconverted (ELISA) between 2 and 4 WPV. Neutralising antibodies appeared 4 WPV in the A-V+ piglets and 14 WPV in the A+V+ piglets. The number of PRRSV-specific IFNγ-secreting cells was significantly higher in A-V+ piglets at 2 and 4 WPV than in A+V+ piglets. These results show that MDAs can affect both post-vaccination humoral and cellular immune responses in piglets. Further studies are required to assess the impact of MDAs on vaccine efficacy following a PRRSV challenge and its ability to reduce viral transmission.

KEYWORDS:

Immune response; Maternal neutralizing antibody; PRRS; Vaccination

PMID:
27527780
DOI:
10.1016/j.vetmic.2016.07.014
[Indexed for MEDLINE]

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