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Int J Environ Res Public Health. 2016 Aug 5;13(8). pii: E793. doi: 10.3390/ijerph13080793.

Twenty Four-Hour Exposure to a 0.12 THz Electromagnetic Field Does Not Affect the Genotoxicity, Morphological Changes, or Expression of Heat Shock Protein in HCE-T Cells.

Author information

1
Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. shin_koyama@rish.kyoto-u.ac.jp.
2
Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. narita.eijirou.4e@kyoto-u.ac.jp.
3
Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. yoko_shimizu@rish.kyoto-u.ac.jp.
4
Department of Electrical & Electronic Engineering, Graduate Schools of Science and Engineering, Tokyo Metropolitan University, 1-1, Hachioji, Tokyo 192-0397, Japan. shiina-takeo@ed.tmu.ac.jp.
5
Department of Electrical & Electronic Engineering, Graduate Schools of Science and Engineering, Tokyo Metropolitan University, 1-1, Hachioji, Tokyo 192-0397, Japan. masao@tmu.ac.jp.
6
Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. shino@rish.kyoto-u.ac.jp.
7
Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. miyakoshi@rish.kyoto-u.ac.jp.

Abstract

To investigate the cellular effects of terahertz (THz) exposure, human corneal epithelial (HCE-T) cells derived from human eye were exposed to 0.12 THz radiation at 5 mW/cm² for 24 h, then the genotoxicity, morphological changes, and heat shock protein (Hsp) expression of the cells were examined. There was no statistically significant increase in the micronucleus (MN) frequency of cells exposed to 0.12 THz radiation compared with sham-exposed controls and incubator controls, whereas the MN frequency of cells treated with bleomycin for 1 h (positive control) did increase significantly. Similarly, there were no significant morphological changes in cells exposed to 0.12 THz radiation compared to sham-exposed controls and incubator controls, and Hsp expression (Hsp27, Hsp70, and Hsp90α) was also not significantly different between the three treatments. These results indicate that exposure to 0.12 THz radiation using the present conditions appears to have no or very little effect on MN formation, morphological changes, and Hsp expression in cells derived from human eye.

KEYWORDS:

cellular genotoxicity; heat shock protein (Hsp); human eye cells; long-term exposure; micronucleus (MN) formation; morphological changes; terahertz (THz)

PMID:
27527204
PMCID:
PMC4997479
DOI:
10.3390/ijerph13080793
[Indexed for MEDLINE]
Free PMC Article

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