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Genet Mol Res. 2016 Aug 12;15(3). doi: 10.4238/gmr.15038330.

Pterostilbene as treatment for severe acute pancreatitis.

Author information

1
Department of Intensive Care, Sir Run Run Shaw Hospital, Medicine School of Zhejiang University, Hangzhou, China.
2
Department of Emergency, Hangzhou Xiasha Hospital, Hangzhou, China.
3
Pediatric Intensive Care Unit, Children's Hospital, Medicine School of Zhejiang University, Hangzhou, China ningbotao122@sina.com.

Abstract

Acute pancreatitis (AP) has a fast onset and progression, which lead to an unfavorable prognosis. Therefore, the development of novel drugs for its treatment is critical. As a homologous derivative of resveratrol, pterostilbene exerts a variety of effects including anti-inflammatory, antioxidant, and antitumor effects. This study investigated the potential of pterostilbene for treatment of severe AP (SAP) and related mechanisms. Effects of pterostilbene were evaluated in a Wistar rat model of AP. Serum levels of amylase (AMY), creatinine (Cr), and alanine aminotransferase (ALT) were quantified. Furthermore, serum levels of tumor necrosis factor (TNF)-a and interleukin (IL)-1b were quantified using enzyme-linked immunosorbent assay. Nuclear factor (NF)-kB expression in pancreatic tissues was quantified by real-time PCR and western blotting. The production of reactive oxygen species (ROS) was determined using a spectrometer, while superoxide dismutase (SOD) activity was assayed. In the AP rat model, the expression of inflammatory markers TNF-a and IL-1b, expression of NF-kB, and serum indices (AMY, Cr, and ALT) increased compared to the corresponding levels in the control group (P < 0.05). Pterostilbene reduced serum levels of TNF-a and IL-1b; decreased NF-kB gene expression, serum indices, and ROS generation; and increased SOD activity in a dose-dependent manner. In conclusion, pterostilbene can alleviate SAP-induced tissue damage by decreasing the inflammatory response and by promoting antioxidation leading to the protection of pancreatic tissues.

PMID:
27525946
DOI:
10.4238/gmr.15038330
[Indexed for MEDLINE]

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