Send to

Choose Destination
JCI Insight. 2016 Jul 7;1(10). pii: 80920. doi: 10.1172/jci.insight.80920.

ISL1 cardiovascular progenitor cells for cardiac repair after myocardial infarction.

Author information

Yale Cardiovascular Research Center, Section of Cardiovascular Medicine, Department of Internal Medicine.
Yale Stem Cell Center.
Department of Laboratory Medicine.
Department of Internal Medicine, Section of Nephrology.
Department of Cell Biology.
Cellular and Molecular Physiology.
Immunobiology, and.
Vascular Biology and Therapeutics Program, Yale University, New Haven, Connecticut, USA.


Cardiovascular progenitor cells (CPCs) expressing the ISL1-LIM-homeodomain transcription factor contribute developmentally to cardiomyocytes in all 4 chambers of the heart. Here, we show that ISL1-CPCs can be applied to myocardial regeneration following injury. We used a rapid 3D methylcellulose approach to form murine and human ISL1-CPC spheroids that engrafted after myocardial infarction in murine hearts, where they differentiated into cardiomyocytes and endothelial cells, integrating into the myocardium and forming new blood vessels. ISL1-CPC spheroid-treated mice exhibited reduced infarct area and increased blood vessel formation compared with control animals. Moreover, left ventricular (LV) contractile function was significantly better in mice transplanted with ISL1-CPCs 4 weeks after injury than that in control animals. These results provide proof-of-concept of a cardiac repair strategy employing ISL1-CPCs that, based on our previous lineage-tracing studies, are committed to forming heart tissue, in combination with a robust methylcellulose spheroid-based delivery approach.

Supplemental Content

Full text links

Icon for American Society for Clinical Investigation Icon for PubMed Central
Loading ...
Support Center