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Cell Chem Biol. 2016 Aug 18;23(8):1014-1022. doi: 10.1016/j.chembiol.2016.07.009. Epub 2016 Aug 11.

Synthetic Lipoteichoic Acid Glycans Are Potential Vaccine Candidates to Protect from Clostridium difficile Infections.

Author information

1
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany; Department of Chemistry and Biochemistry, Freie Universität Berlin, 14195 Berlin, Germany.
2
Mikrobiologisches Institut ? Klinische Mikrobiologie, Immunologie und Hygiene, Universitätsklinikum Erlangen and Friedrich-Alexander Universität Erlangen-Nürnberg, 91054 Erlangen, Germany.
3
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
4
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany. Electronic address: achakkum@its.jnj.com.
5
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany; Department of Chemistry and Biochemistry, Freie Universität Berlin, 14195 Berlin, Germany. Electronic address: peter.seeberger@mpikg.mpg.de.

Abstract

Infections with Clostridium difficile increasingly cause morbidity and mortality worldwide. Bacterial surface glycans including lipoteichoic acid (LTA) were identified as auspicious vaccine antigens to prevent colonization. Here, we report on the potential of synthetic LTA glycans as vaccine candidates. We identified LTA-specific antibodies in the blood of C. difficile patients. Therefore, we evaluated the immunogenicity of a semi-synthetic LTA-CRM197 glycoconjugate. The conjugate elicited LTA-specific antibodies in mice that recognized natural LTA epitopes on the surface of C. difficile bacteria and inhibited intestinal colonization of C. difficile in mice in vivo. Our findings underscore the promise of synthetic LTA glycans as C. difficile vaccine candidates.

KEYWORDS:

Clostridium difficile; LTA; carbohydrates; lipoteichoic acid; vaccine

PMID:
27524293
DOI:
10.1016/j.chembiol.2016.07.009
[Indexed for MEDLINE]
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