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Biochem Biophys Res Commun. 2016 Oct 7;479(1):1-4. doi: 10.1016/j.bbrc.2016.08.055. Epub 2016 Aug 11.

Siglec-F is a novel intestinal M cell marker.

Author information

1
Food and Health Institute Strategic Program, Institute of Food Research, Norwich, UK.
2
Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA, USA.
3
Department of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA, USA.
4
Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA, USA; Department of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA, USA; Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA, USA. Electronic address: jpaulson@scripps.edu.
5
Food and Health Institute Strategic Program, Institute of Food Research, Norwich, UK; Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA, USA. Electronic address: norihito.kawasaki@ifr.ac.uk.

Abstract

Intestinal microfold (M) cells are epithelial cells primarily present on Peyer's patches (PPs) in the small intestine. The ability of M cells to shuttle antigens into the PP for appropriate immune responses makes M cells a target for next-generation oral vaccine delivery. In this regard, discovery of M cell-specific receptors are of great interest, which could act as molecular tags for targeted delivery of cargo to M cells. Here, using a monoclonal antibody we generated to the Sialic acid-binding immunoglobulin-like lectin F (Siglec-F), we show that Siglec-F is expressed on mouse M cells in the small intestine. Immunohistochemical analysis of the PP tissue sections shows that Siglec-F is expressed on the surface of the M cell membrane exposed to the intestinal lumen. Anti-Siglec-F antibody injected into the mouse small intestine bound to M cells, demonstrating the potential to target M cells via Siglec-F.

KEYWORDS:

M cell; Oral vaccine; Peyer's patch; Siglec

PMID:
27524237
PMCID:
PMC5045273
DOI:
10.1016/j.bbrc.2016.08.055
[Indexed for MEDLINE]
Free PMC Article

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