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Mater Sci Eng C Mater Biol Appl. 2016 Nov 1;68:251-257. doi: 10.1016/j.msec.2016.05.046. Epub 2016 May 14.

Preparation, characterization and biological evaluation of curcumin loaded alginate aldehyde-gelatin nanogels.

Author information

1
Department of Chemistry, Indian Institute of Space Science and Technology (IIST), Valiamala, Thiruvananthapuram, Kerala 695 547, India. Electronic address: sarikapaithal@gmail.com.
2
Department of Chemistry, Indian Institute of Space Science and Technology (IIST), Valiamala, Thiruvananthapuram, Kerala 695 547, India. Electronic address: nirmala@iist.ac.in.
3
Tissue Culture Laboratory, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojappura, Thiruvananthapuram, Kerala 695 012, India. Electronic address: anilkumarpr@sctimst.ac.in.
4
Tissue Culture Laboratory, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojappura, Thiruvananthapuram, Kerala 695 012, India. Electronic address: kdeeps3@gmail.com.

Abstract

Curcumin, a natural polyphenol exhibits chemopreventive and chemotherapeutic activities towards cancer. In order to improve the bioavailability and therapeutic efficacy, curcumin is encapsulated in alginate aldehyde-gelatin (Alg Ald-Gel) nanogels. Alginate aldehyde-gelatin nanogels are prepared by inverse miniemulsion technique. Physicochemical properties of the curcumin loaded nanogels are evaluated by, Dynamic light scattering (DLS), NMR spectroscopy and Scanning electron microscopy (SEM). Curcumin loaded nanogels show hydrodynamic diameter of 431±8nm and a zeta potential of -36±4mV. The prepared nanogels exhibit an encapsulation efficiency of 72±2%. In vitro drug release studies show a controlled release of curcumin from nanogels over a period of 48h. Hemocompatibility and cytocompatibility of the nanogels are evaluated. Bare nanogels are cytocompatible and curcumin loaded nanogels induce anticancer activity towards MCF-7 cells. In vitro cellular uptake of the curcumin loaded nanogels using confocal laser scanning microscopy (CLSM) confirms the uptake of nanogels in MCF-7 cells. Hence, the developed nanogel system can be a suitable candidate for curcumin delivery to cancer cells.

KEYWORDS:

Alginate aldehyde; Curcumin; Drug delivery; Gelatin; Nanogels

PMID:
27524019
DOI:
10.1016/j.msec.2016.05.046
[Indexed for MEDLINE]

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