Format

Send to

Choose Destination
Insect Biochem Mol Biol. 2016 Sep;76:118-147. doi: 10.1016/j.ibmb.2016.07.005. Epub 2016 Aug 12.

Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth, Manduca sexta.

Author information

1
Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, KS, 66506, USA. Electronic address: Kanost@ksu.edu.
2
Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK, 74078, USA.
3
Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA.
4
Boyce Thompson Institute at Cornell University, Tower Road, Ithaca, NY, 14853, USA.
5
KSU Bioinformatics Center, Division of Biology, Kansas State University, Manhattan, KS, 66506, USA.
6
Biological Sciences Department, University of Rhode Island, Kingston, RI, 02881, USA.
7
Max Planck Institute for Chemical Ecology, Department of Evolutionary Neuroethology, Hans-Knoell-Strasse, 8, D-07745, Jena, Germany.
8
Department of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745, Jena, Germany.
9
Hawkesbury Institute for the Environment, Western Sydney University, Richmond, NSW, 2753, Australia.
10
Human Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
11
Department of Ecology and Evolutionary Biology, Univ. Kansas, Lawrence, KS, 66045, USA.
12
Department of Genetic Medicine and Development, University of Geneva Medical School, rue Michel-Servet 1, 1211, Geneva, Switzerland; Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211, Geneva, Switzerland; Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, 32 Vassar Street, Cambridge, MA, 02139, USA; The Broad Institute of MIT and Harvard, Cambridge, 415 Main Street, MA, 02142, USA.
13
Departament de Genètica and Institut de Recerca de la Biodiversitat (IRBio), Universitat de Barcelona, Barcelona, Spain.
14
Department of Entomology, China Agricultural University, Beijing, China.
15
Division of Biology, Kansas State University, Manhattan, KS, 66506, USA.
16
Institut de Recherche sur la Biologie de l'Insecte, UMR CNRS 7261, UFR Sciences et Techniques, Université François-Rabelais, Tours, France.
17
Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, KS, 66506, USA.
18
CSIRO Land and Water, Clunies Ross St, Acton, ACT, 2601, Australia.
19
Department of Zoology, South Parks Road, Oxford, UK.
20
Centre of Systems Biology, Biomedical Research Foundation, Academy of Athens, Athens, Greece.
21
CSIRO Health and Biosecurity, Clunies Ross St, Acton, ACT, 2601, Australia.
22
Center for Functional and Comparative Insect Genomics, Department of Biology, University of Copenhagen, Universitetsparken 15, DK-21oo, Copenhagen, Denmark.
23
Department of Biology, Barnard College, Columbia University, 3009 Broadway, New York, NY, 10027, USA.
24
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, 400715, China.
25
Department of Biology, Reed College, Portland, OR, 97202, USA.
26
Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Bioresources Project Group, Winchesterstrasse 2, 35394, Gießen, Germany.
27
College of Chemistry, Chemical Engineering, and Biotechnology, Donghua University, Shanghai, 201620, China.
28
College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, 712100, China.
29
McDermott Center for Human Growth and Development, UT Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX, 75390, USA.
30
Department of Biological Sciences, University of Southern Maine, Portland, ME, 04104, USA.
31
Department of Ecology and Evolutionary Biology, University of California, Irvine, CA, 92697, USA.
32
School of Plant Protection, Anhui Agricultural University, Hefei, Anhui, China.
33
Department of Biology, University of Rochester, Rochester, NY, 14627, USA.
34
Department of Marine and Environmental Sciences, Northeastern University, Boston, MA, 02115, USA.
35
Department of Entomology, Cornell University, New York State Agricultural Experiment Station, Geneva, NY, 14456, USA.
36
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
37
Institut fuer Biologie, Universitaet Luebeck, D-23538, Luebeck, Germany.
38
Neuroscience Program, Hamilton College, Clinton, NY, 13323, USA.
39
Institute of Zoology, University of Hamburg, Germany.
40
Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
41
Department of Cell Biology and Biophysics, Faculty of Biology, University of Athens, Athens, Greece.
42
Department of Entomology, University of Wisconsin, Madison, USA.
43
Biology Department and Neuroscience Program, Hamilton College, Clinton, NY, 13323, USA.
44
Dept. Entomology, North Carolina State Univ., Raleigh, NC, 27695, USA.
45
University of Siegen, School of Natural Sciences and Engineering, Institute of Biology - Molecular Biology, Adolf-Reichwein-Strasse. 2, AR-C3010, 57076 Siegen, Germany.
46
Department of Integrative Biosciences, School of Dentistry, BRB421, L595, Oregon Health & Science University, 3181 SW Sam Jackson Park Rd., Portland, OR, 97239, USA.
47
Department of Genetics, University of Cambridge, Downing St, Cambridge, CB2 3EH, UK.
48
Department of Entomology, Kansas State University, Manhattan, KS, 66506, USA.
49
Department of Biology, University of Massachusetts, Amherst, MA, 01003, USA.
50
Department of Biology, Northeastern University, Boston, MA, 02115, USA.
51
Department of Biology, College of Charleston, Charleston, SC, 29424, USA.
52
Institute for Insect Biotechnology, Justus-Liebig-University, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany.
53
Department of Biological Sciences, University of South Carolina, Columbia, SC, 29205, USA.
54
University of Missouri-Kansas City, 5007 Rockhill Road, Kansas City, MO, 64110, USA.
55
Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK.

Abstract

Manduca sexta, known as the tobacco hornworm or Carolina sphinx moth, is a lepidopteran insect that is used extensively as a model system for research in insect biochemistry, physiology, neurobiology, development, and immunity. One important benefit of this species as an experimental model is its extremely large size, reaching more than 10 g in the larval stage. M. sexta larvae feed on solanaceous plants and thus must tolerate a substantial challenge from plant allelochemicals, including nicotine. We report the sequence and annotation of the M. sexta genome, and a survey of gene expression in various tissues and developmental stages. The Msex_1.0 genome assembly resulted in a total genome size of 419.4 Mbp. Repetitive sequences accounted for 25.8% of the assembled genome. The official gene set is comprised of 15,451 protein-coding genes, of which 2498 were manually curated. Extensive RNA-seq data from many tissues and developmental stages were used to improve gene models and for insights into gene expression patterns. Genome wide synteny analysis indicated a high level of macrosynteny in the Lepidoptera. Annotation and analyses were carried out for gene families involved in a wide spectrum of biological processes, including apoptosis, vacuole sorting, growth and development, structures of exoskeleton, egg shells, and muscle, vision, chemosensation, ion channels, signal transduction, neuropeptide signaling, neurotransmitter synthesis and transport, nicotine tolerance, lipid metabolism, and immunity. This genome sequence, annotation, and analysis provide an important new resource from a well-studied model insect species and will facilitate further biochemical and mechanistic experimental studies of many biological systems in insects.

KEYWORDS:

Innate immunity; Insect; Insect biochemistry; Lepidoptera; Moth; Synteny; Tobacco hornworm

PMID:
27522922
PMCID:
PMC5010457
DOI:
10.1016/j.ibmb.2016.07.005
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center