Format

Send to

Choose Destination
Immunity. 2016 Aug 16;45(2):442-56. doi: 10.1016/j.immuni.2016.07.007. Epub 2016 Aug 9.

A High-Dimensional Atlas of Human T Cell Diversity Reveals Tissue-Specific Trafficking and Cytokine Signatures.

Author information

1
Agency for Science, Technology and Research (A(∗)STAR), Singapore Immunology Network (SIgN), Singapore 138648, Singapore. Electronic address: michael.thomas.wong@merck.com.
2
Division of Gastroenterology and Hepatology, University Medicine Cluster, National University Health System, Singapore 119228, Singapore.
3
Division of Colorectal Surgery, University Surgical Center, National University Health System, Singapore 119228, Singapore.
4
Agency for Science, Technology and Research (A(∗)STAR), Singapore Immunology Network (SIgN), Singapore 138648, Singapore.
5
Agency for Science, Technology and Research (A(∗)STAR), Singapore Immunology Network (SIgN), Singapore 138648, Singapore; School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore.
6
Department of Otolaryngology, KK Women's and Children's Hospital, Singapore 229899, Singapore.
7
Department of Plastic, Reconstructive and Aesthetic Surgery, Singapore General Hospital, Singapore 169608, Singapore.
8
Department of Pathology, Singapore General Hospital, Singapore 169856, Singapore.
9
Department of Hepato-pancreato-biliary and Transplant Surgery, Singapore General Hospital, Singapore 169856, Singapore.
10
Department of Medical Oncology, National Cancer Centre, Singapore 169610, Singapore.
11
Department of Surgery, National University of Singapore, Singapore 119228, Singapore.
12
Department of General Surgery, Tan Tock Seng Hospital, Singapore 308433, Singapore.
13
Department of Reproductive Medicine, KK Women's and Children's Hospital, Singapore 229899, Singapore.
14
Agency for Science, Technology and Research (A(∗)STAR), Singapore Immunology Network (SIgN), Singapore 138648, Singapore; Emerging Infectious Diseases (EID) Program, Duke-NUS Medical School, Singapore 169857, Singapore.
15
Agency for Science, Technology and Research (A(∗)STAR), Singapore Immunology Network (SIgN), Singapore 138648, Singapore. Electronic address: evan_newell@immunol.a-star.edu.sg.

Abstract

Depending on the tissue microenvironment, T cells can differentiate into highly diverse subsets expressing unique trafficking receptors and cytokines. Studies of human lymphocytes have primarily focused on a limited number of parameters in blood, representing an incomplete view of the human immune system. Here, we have utilized mass cytometry to simultaneously analyze T cell trafficking and functional markers across eight different human tissues, including blood, lymphoid, and non-lymphoid tissues. These data have revealed that combinatorial expression of trafficking receptors and cytokines better defines tissue specificity. Notably, we identified numerous T helper cell subsets with overlapping cytokine expression, but only specific cytokine combinations are secreted regardless of tissue type. This indicates that T cell lineages defined in mouse models cannot be clearly distinguished in humans. Overall, our data uncover a plethora of tissue immune signatures and provide a systemic map of how T cell phenotypes are altered throughout the human body.

PMID:
27521270
DOI:
10.1016/j.immuni.2016.07.007
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center