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Plant Cell Physiol. 2016 Nov;57(11):2255-2268. Epub 2016 Aug 12.

Combination of Synthetic Chemistry and Live-Cell Imaging Identified a Rapid Cell Division Inhibitor in Tobacco and Arabidopsis thaliana.

Author information

1
Institute of Transformative Bio-Molecules (ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601 Japan m-ueda@itbm.nagoya-u.ac.jp mnambo@itbm.nagoya-u.ac.jp.
2
These authors contributed equally to this work.
3
Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602 Japan.
4
JST, ERATO Higashiyama Live-Holonics Project, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602 Japan.
5
Institute of Transformative Bio-Molecules (ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601 Japan.
6
Laboratory of Animal Physiology, Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601 Japan.
7
Laboratory of Plant Growth Regulation, Graduate School of Biological Sciences, Nara Institute of Science and Technology (NAIST), Takayama-cho 8916-5, Ikoma, Nara, 630-0192 Japan.
8
JST, CREST, Takayama-cho 8916-5, Ikoma, Nara, 630-0192 Japan.

Abstract

Cell proliferation is crucial to the growth of multicellular organisms, and thus the proper control of cell division is important to prevent developmental arrest or overgrowth. Nevertheless, tools for controlling cell proliferation are still poor in plant. To develop novel tools, we focused on a specific compound family, triarylmethanes, whose members show various antiproliferative activities in animals. By combining organic chemistry to create novel and diverse compounds containing the triarylmethyl moiety and biological screens based on live-cell imaging of a fluorescently labeled tobacco Bright Yellow-2 (BY-2) culture cell line (Nicotiana tabacum), we isolated (3-furyl)diphenylmethane as a strong but partially reversible inhibitor of plant cell division. We also found that this agent had efficient antiproliferative activity in developing organs of Arabidopsis thaliana without causing secondary defects in cell morphology, and induced rapid cell division arrest independent of the cell cycle stage. Given that (3-furyl)diphenylmethane did not affect the growth of a human cell line (HeLa) and a budding yeast (Saccharomyces cerevisiae), it should act specifically on plants. Taking our results together, we propose that the combination of desired chemical synthesis and detailed biological analysis is an effective tool to create novel drugs, and that (3-furyl)diphenylmethane is a specific antiproliferative agent for plants.

KEYWORDS:

Arabidopsis thaliana ; Cell division inhibitor; Live-cell imaging; Organic chemistry; Tobacco BY-2 cell; Triarylmethane

PMID:
27519314
DOI:
10.1093/pcp/pcw140
[Indexed for MEDLINE]

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