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Methods Mol Biol. 2016;1473:123-34. doi: 10.1007/978-1-4939-6346-1_13.

Correction of Microplate Data from High-Throughput Screening.

Author information

1
National Center for Advancing Translational Sciences, National Institutes of Health, Building B, MSC: 3370, 9800 Medical Center Drive, Bethesda, MD, 20892, USA. wangyuh@mail.nih.gov.
2
National Center for Advancing Translational Sciences, National Institutes of Health, Building B, MSC: 3370, 9800 Medical Center Drive, Bethesda, MD, 20892, USA.

Abstract

High-throughput screening (HTS) makes it possible to collect cellular response data from a large number of cell lines and small molecules in a timely and cost-effective manner. The errors and noises in the microplate-formatted data from HTS have unique characteristics, and they can be generally grouped into three categories: run-wise (temporal, multiple plates), plate-wise (background pattern, single plate), and well-wise (single well). In this chapter, we describe a systematic solution for identifying and correcting such errors and noises, mainly basing on pattern recognition and digital signal processing technologies.

KEYWORDS:

Concentration response; Data correction; HTS; In vitro assay

PMID:
27518630
DOI:
10.1007/978-1-4939-6346-1_13
[Indexed for MEDLINE]

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