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Methods Mol Biol. 2016;1473:77-85. doi: 10.1007/978-1-4939-6346-1_9.

High-Throughput and High-Content Micronucleus Assay in CHO-K1 Cells.

Author information

1
National Center for Advancing Translational Sciences, National Institutes of Health, Building C, MSC: 3375, 9800 Medical Center Drive, Bethesda, MD, 20892, USA.
2
National Center for Advancing Translational Sciences, National Institutes of Health, Building C, MSC: 3375, 9800 Medical Center Drive, Bethesda, MD, 20892, USA. mxia@mail.nih.gov.

Abstract

Visualization of micronuclei induction by chemicals and drugs enables measurement of possible compound genotoxicity. A loss of entire chromosome or a fragment of chromosome can lead to formation of micronuclei (MNi). The in vitro micronucleus assay can be conducted using nuclear dyes with high-content imaging platforms. This chapter describes the cytochalasin block method of measuring micronuclei in CHO-K1 cell lines.

KEYWORDS:

Genotoxicity; High-content imaging; Micronuclei

PMID:
27518626
DOI:
10.1007/978-1-4939-6346-1_9
[Indexed for MEDLINE]

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