Format

Send to

Choose Destination
Angew Chem Int Ed Engl. 2016 Sep 5;55(37):11242-6. doi: 10.1002/anie.201605151. Epub 2016 Aug 11.

Gold-Induced Fibril Growth: The Mechanism of Surface-Facilitated Amyloid Aggregation.

Author information

1
Chemistry Department, Leibniz Institute of Surface Modification (IOM) Leipzig e.V., Permoserstrasse 15, 04318, Leipzig, Germany.
2
Faculty of Chemistry and Mineralogy, University of Leipzig, Linnéstrasse 3, 04193, Leipzig, Germany.
3
Chemistry Department, Leibniz Institute of Surface Modification (IOM) Leipzig e.V., Permoserstrasse 15, 04318, Leipzig, Germany. bernd.abel@iom-leipzig.de.
4
Faculty of Chemistry and Mineralogy, University of Leipzig, Linnéstrasse 3, 04193, Leipzig, Germany. bernd.abel@iom-leipzig.de.
5
Chemistry Department, Leibniz Institute of Surface Modification (IOM) Leipzig e.V., Permoserstrasse 15, 04318, Leipzig, Germany. hrissel@gwdg.de.
6
Department of Theoretical Physics, Georg-August-University, Friedrich-Hund-Platz 1, 37077, Göttingen, Germany. hrissel@gwdg.de.

Abstract

The question of how amyloid fibril formation is influenced by surfaces is crucial for a detailed understanding of the process in vivo. We applied a combination of kinetic experiments and molecular dynamics simulations to elucidate how (model) surfaces influence fibril formation of the amyloid-forming sequences of prion protein SUP35 and human islet amyloid polypeptide. The kinetic data suggest that structural reorganization of the initial peptide corona around colloidal gold nanoparticles is the rate-limiting step. The molecular dynamics simulations reveal that partial physisorption to the surface results in the formation of aligned monolayers, which stimulate the formation of parallel, critical oligomers. The general mechanism implies that the competition between the underlying peptide-peptide and peptide-surface interactions must strike a balance to accelerate fibril formation.

KEYWORDS:

Ramachandran plots; SUP35; citrate-covered gold nanoparticles; hIAPP; molecular dynamics simulations

PMID:
27513605
PMCID:
PMC5113782
DOI:
10.1002/anie.201605151
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center