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J Biomed Mater Res A. 2017 Jan;105(1):62-72. doi: 10.1002/jbm.a.35863. Epub 2016 Aug 27.

Competitive colonization of prosthetic surfaces by staphylococcus aureus and human cells.

Author information

1
Otorhinolaryngology-Head and Neck Surgery, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
2
Medicine, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
3
ORTON Research Institute, Helsinki, Finland.
4
ORTON Orthopedic Hospital, Helsinki, Finland.
5
Rheumatology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
6
Clinical Microbiology, IIS-Fundación Jiménez Díaz, Madrid, Spain.

Abstract

Implantation of a biomaterial provides an adhesion substratum both to host cell integration and to contaminating bacteria. We studied simultaneous competitive adhesion of Staphylococcus aureus in serial 1:10 dilutions of 108 colony forming units (CFU)/mL and human osteogenic sarcoma (SaOS-2) or primary osteoblast (hOB) cells, both 1x105 cells/mL, to the surfaces of titanium, polydimethylsiloxane and polystyrene. The bacterial adherence and human cell proliferation, cytotoxicity and production of reactive oxygen species (ROS) were studied using fluorometric (fluorescent microscopy and flow cytometry) and colorimetric methods (MTT, LDH and crystal violet). The bacterial cell viability was also evaluated using the drop plate method. The presence of bacteria resulted in reduced adherence of human cells to the surface of the biomaterials, increased production of ROS, and into increased apoptosis. On the other hand, the presence of either type of human cells was associated with a reduction of bacterial colonization of the biomaterial with Staphylococcus aureus. These results suggest that increasing colonization of the biomaterial surface in vitro by one negatively affects colonization by the other. Host cell integration to an implant surface reduces bacterial contamination, which opens novel opportunities for the design of infection-resistant biomaterials in current implantology and future regenerative medicine.

KEYWORDS:

bacterial adherence; biofilm; biomaterial; cellular oxidative stress; race for the surface

PMID:
27513443
DOI:
10.1002/jbm.a.35863
[Indexed for MEDLINE]

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