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J Genomics. 2016 Jul 26;4:19-22. doi: 10.7150/jgen.16082. eCollection 2016.

External RNA Controls Consortium Beta Version Update.

Author information

1
Section of Developmental Genomics, Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
2
Joint Initiative for Metrology in Biology, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.

Abstract

Spike-in RNAs are valuable controls for a variety of gene expression measurements. The External RNA Controls Consortium developed test sets that were used in a number of published reports. Here we provide an authoritative table that summarizes, updates, and corrects errors in the test version that ultimately resulted in the certified Standard Reference Material 2374. We have noted existence of anti-sense RNA controls in the material, corrected sub-pool memberships, and commented on control RNAs that displayed inconsistent behavior.

KEYWORDS:

ERCC; NIST standard reference materials.; external RNA controls; spike-in controls

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