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Indian J Orthop. 2016 Jul-Aug;50(4):421-6. doi: 10.4103/0019-5413.185608.

N-terminal telopeptides of type I collagen and bone mineral density for early diagnosis of nonunion: An experimental study in rabbits.

Author information

1
Department of Orthopedic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China; Department of Orthopedic Surgery, Hainan Provincial People's Hospital, Haikou 570311, Hainan Province, China.
2
Department of Orthopedic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
3
Department of Orthopedic Surgery, Hainan Provincial People's Hospital, Haikou 570311, Hainan Province, China.

Abstract

BACKGROUND:

The diagnosis and treatment of bone nonunion have been studied extensively. Diagnosis and treatment of nonunion are mainly performed based on the interpretation of clinico-radiographic findings, which depend on the clinician's experience and the degree of bone callus formation during the fracture-healing process. However, resolution may be compromised when the bone mineral content is <25%. A feasible method of monitoring bone-healing is therefore needed. We monitored a rabbit model of bone nonunion by regular radiographic examinations, QCT detection, and biomarker concentrations.

MATERIALS AND METHODS:

Twenty purebred New Zealand rabbits (10 male and 10 female, 5-6 months of age, 2.5-3.0 kg) were divided into bone defect Group (I) that 10 left radius bones underwent resection of 1.5 cm of mid-radius bone and bone fracture Group (II) that another 10 left radius bones underwent only mid-radius fracture. Quantitative computed tomography detection of bone mineral density (BMD) and serum markers of bone formation (osteocalcin [OC], bone-specific alkaline phosphatase) and bone resorption (C- and N-terminal telopeptides of type I collagen (NTX) and tartrate-resistant acid phosphatase 5b) were assayed. There are twenty rabbits (10 male and 10 females). The age was 5-6 months weighing 2.5-3.0 kg). The defect was created in middle 1/3 radius in 10 rabbits and fracture was created in middle 1/3 radius of 10 rabbits.

RESULTS:

BMD and NTX concentrations were significantly lower at 5 weeks postoperatively compared to the preoperative values and were significantly different between the two groups. OC showed no significant difference before and after surgery.

CONCLUSIONS:

BMD and NTX concentrations may be useful for early detection of bone nonunion in rabbits.

KEYWORDS:

Biomarkers; N-terminal telopeptide of type I collagen; Osteoporosis; biomarkers; bone fractures; bone mineral density; bone-specific alkaline phosphatase; nonunion; tartrate-resistant acid phosphatase 5b

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