Format

Send to

Choose Destination
Sci Rep. 2016 Aug 11;6:31313. doi: 10.1038/srep31313.

CircRNA-protein complexes: IMP3 protein component defines subfamily of circRNPs.

Author information

1
Institute of Biochemistry, University of Giessen, Heinrich-Buff-Ring 17, D-35392 Giessen, Germany.
2
Biochemistry I, University of Regensburg, Universitätsstrasse 31, D-93053 Regensburg, Germany.

Abstract

Circular RNAs (circRNAs) constitute a new class of noncoding RNAs in higher eukaryotes generated from pre-mRNAs by alternative splicing. Here we investigated in mammalian cells the association of circRNAs with proteins. Using glycerol gradient centrifugation, we characterized in cell lysates circRNA-protein complexes (circRNPs) of distinct sizes. By polysome-gradient fractionation we found no evidence for efficient translation of a set of abundant circRNAs in HeLa cells. To identify circRNPs with a specific protein component, we focused on IMP3 (IGF2BP3, insulin-like growth factor 2 binding protein 3), a known tumor marker and RNA-binding protein. Combining RNA-seq analysis of IMP3-co-immunoprecipitated RNA and filtering for circular-junction reads identified a set of IMP3-associated circRNAs, which were validated and characterized. In sum, our data suggest that specific circRNP families exist defined by a common protein component. In addition, this provides a general approach to identify circRNPs with a given protein component.

PMID:
27510448
PMCID:
PMC4980667
DOI:
10.1038/srep31313
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center