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Int J Environ Res Public Health. 2016 Aug 8;13(8). pii: E802. doi: 10.3390/ijerph13080802.

Effects of Long-Term Exposure to 60 GHz Millimeter-Wavelength Radiation on the Genotoxicity and Heat Shock Protein (Hsp) Expression of Cells Derived from Human Eye.

Author information

1
Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. shin_koyama@rish.kyoto-u.ac.jp.
2
Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. narita.eijirou.4e@kyoto-u.ac.jp.
3
Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. yoko_shimizu@rish.kyoto-u.ac.jp.
4
Department of Electrical & Electronic Engineering, Graduate Schools of Science and Engineering, Tokyo Metropolitan University, 1-1, Hachioji, Tokyo 192-0397, Japan. y_suzuki@tmu.ac.jp.
5
Department of Electrical & Electronic Engineering, Graduate Schools of Science and Engineering, Tokyo Metropolitan University, 1-1, Hachioji, Tokyo 192-0397, Japan. shiina-takeo@ed.tmu.ac.jp.
6
Department of Electrical & Electronic Engineering, Graduate Schools of Science and Engineering, Tokyo Metropolitan University, 1-1, Hachioji, Tokyo 192-0397, Japan. masao@tmu.ac.jp.
7
Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. shino@rish.kyoto-u.ac.jp.
8
Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. miyakoshi@rish.kyoto-u.ac.jp.

Abstract

Human corneal epithelial (HCE-T) and human lens epithelial (SRA01/04) cells derived from the human eye were exposed to 60 gigahertz (GHz) millimeter-wavelength radiation for 24 h. There was no statistically significant increase in the micronucleus (MN) frequency in cells exposed to 60 GHz millimeter-wavelength radiation at 1 mW/cm² compared with sham-exposed controls and incubator controls. The MN frequency of cells treated with bleomycin for 1 h provided positive controls. The comet assay, used to detect DNA strand breaks, and heat shock protein (Hsp) expression also showed no statistically significant effects of exposure. These results indicate that exposure to millimeter-wavelength radiation has no effect on genotoxicity in human eye cells.

KEYWORDS:

cellular genotoxicity; comet assay; heat shock protein; human eye cells; long-term exposure; micronucleus formation; millimeter-waves

PMID:
27509516
PMCID:
PMC4997488
DOI:
10.3390/ijerph13080802
[Indexed for MEDLINE]
Free PMC Article

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