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Methods Mol Biol. 2016;1476:167-75. doi: 10.1007/978-1-4939-6361-4_12.

Induction and Purification of C. difficile Phage Tail-Like Particles.

Author information

1
Department of Surgery, Division of Colorectal Surgery, The Pennsylvania State University College of Medicine, 500 University Drive, H-137, 850, Hershey, PA, 17033, USA. jhegarty1@hmc.psu.edu.
2
Department of Surgery, Division of Colorectal Surgery, The Pennsylvania State University College of Medicine, 500 University Drive, H-137, 850, Hershey, PA, 17033, USA.
3
Department of Medicine, The Pennsylvania State University College of Medicine, Hershey, PA, USA.
4
Department of Microbiology & Immunology, The Pennsylvania State University College of Medicine, Hershey, PA, USA.
5
Department of Neural and Behavioral Sciences, The Pennsylvania State University College of Medicine, Hershey, PA, USA.
6
Department of Surgery, Division of Colorectal Surgery, The Pennsylvania State University College of Medicine, 500 University Drive, H-137, 850, Hershey, PA, 17033, USA. dstewart@hmc.psu.edu.

Abstract

Due to the inherent limitations of conventional antibiotics for the treatment of C. difficile infection (CDI), there is a growing interest in the development of alternative treatment strategies. Both bacteriophages and R-type bacteriocins, also known as phage tail-like particles (PTLPs), show promise as potential antibacterial alternatives for treating CDI. Similar to bacteriophages, but lacking a viral capsid and genome, PTLPs remain capable of killing target bacteria. Here we describe our experience in the induction and purification of C. difficile PTLPs. These methods have been optimized to allow production of concentrated, non-contractile, and non-aggregated samples for both sensitivity testing and structural electron microscopy studies.

KEYWORDS:

Clostridium difficile; Induction; Microscopy; Phage tail-like particles; Purification

PMID:
27507340
DOI:
10.1007/978-1-4939-6361-4_12
[Indexed for MEDLINE]

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