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Mult Scler. 2017 May;23(6):802-809. doi: 10.1177/1352458516664210. Epub 2016 Aug 8.

Increased ex vivo antigen presentation profile of B cells in multiple sclerosis.

Author information

1
Laboratory of Neuroimmunology, Neuroscience Research Centre, Department of Clinical Neurosciences, Lausanne University Hospital, Lausanne, Switzerland.
2
Bioinformatics Core Facility, SIB Swiss Institute of Bioinformatics, Lausanne, Switzerland.
3
Bioinformatics Core Facility, SIB Swiss Institute of Bioinformatics, Lausanne, Switzerland/Ludwig Center for Cancer Research and Department of Oncology, University of Lausanne, Lausanne, Switzerland.
4
Service of Neurology, Department of Clinical Neurosciences, Lausanne University Hospital, Lausanne, Switzerland.
5
Laboratory of Neuroimmunology, Neuroscience Research Centre, Department of Clinical Neurosciences, Lausanne University Hospital, Lausanne, Switzerland/Service of Neurology, Department of Clinical Neurosciences, Lausanne University Hospital, Lausanne, Switzerland.

Abstract

BACKGROUND:

Multiple sclerosis (MS) is thought to be T cell mediated but the mechanisms eliciting such a dysregulated adaptative immune response remain enigmatic.

OBJECTIVE:

To examine the activation profile of antigen-presenting cells (APCs) in MS.

METHODS:

A total of 98 study subjects were enrolled including patients suffering from relapsing-remitting, secondary- and primary-progressive (PP) MS, other inflammatory neurological diseases, and healthy controls. Blood monocytes and B cells were stimulated using specific ligands of toll-like receptors (TLRs) or inflammasomes or Epstein-Barr virus (EBV) particles. Their activation profile was determined before or after stimulation by flow cytometry (CD40, CD80, CD83, CD86, and human leukocyte antigen-antigen D related (HLA-DR)) and Luminex assay, measuring the concentration of eight cytokines in culture supernatants. Differences among groups were assessed in a linear model framework.

RESULTS:

We demonstrate that relapsing MS patients exhibit an increased expression of HLA-DR and CD40 ex vivo, mostly at the surface of B cells. Specific stimulations of TLR or inflammasomes enhance the expression of components of the immunological synapse and the cytokine secretion but without differences between categories of study subjects.

CONCLUSION:

These data suggest that the activation profile of B cells is increased in MS. However, the perception of the danger signal by B lymphocytes and monocytes does not seem to be different in MS patients as compared to control subjects.

KEYWORDS:

B cells; Multiple sclerosis; antigen-presenting cell activation profile; cytokine secretion

PMID:
27503907
DOI:
10.1177/1352458516664210
[Indexed for MEDLINE]

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