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Elife. 2016 Aug 9;5. pii: e16550. doi: 10.7554/eLife.16550.

Physical association between a novel plasma-membrane structure and centrosome orients cell division.

Author information

1
Division of Morphogenesis, National Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki, Japan.
2
Laboratoire de Biologie du Développement de Villefranche-sur-mer UMR7009, Observatoire Océanologique, Sorbonne Universités, UPMC Université Paris 06, CNRS, Villefranche-sur-Mer, France.
3
National Institute for Physiological Sciences, National Institutes of Natural Sciences, Okazaki, Japan.
4
Department of Basic Biology, School of Life Science, The Graduate University for Advanced Studies, Okazaki, Japan.

Abstract

In the last mitotic division of the epidermal lineage in the ascidian embryo, the cells divide stereotypically along the anterior-posterior axis. During interphase, we found that a unique membrane structure invaginates from the posterior to the centre of the cell, in a microtubule-dependent manner. The invagination projects toward centrioles on the apical side of the nucleus and associates with one of them. Further, a cilium forms on the posterior side of the cell and its basal body remains associated with the invagination. A laser ablation experiment suggests that the invagination is under tensile force and promotes the posterior positioning of the centrosome. Finally, we showed that the orientation of the invaginations is coupled with the polarized dynamics of centrosome movements and the orientation of cell division. Based on these findings, we propose a model whereby this novel membrane structure orchestrates centrosome positioning and thus the orientation of cell division axis.

KEYWORDS:

C. intestinalis; Ciliary positioning; cell biology; developmental biology; spindle orientation; stem cells

PMID:
27502556
PMCID:
PMC4978527
DOI:
10.7554/eLife.16550
[Indexed for MEDLINE]
Free PMC Article

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