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Plant J. 2016 Dec;88(6):947-962. doi: 10.1111/tpj.13296. Epub 2016 Oct 5.

Jasmonate-mediated stomatal closure under elevated CO2 revealed by time-resolved metabolomics.

Author information

1
Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL, 32610, USA.
2
Department of Biology, Genetics Institute, University of Florida, Gainesville, FL, 32610, USA.
3
Thermo Fisher Scientific, 1400 Northpoint Parkway, West Palm Beach, FL, 33407, USA.
4
Samuel Roberts Noble Foundation, Ardmore, OK, 73401, USA.
5
Chemistry Research Unit, Agricultural Research Service, United States Department of Agriculture, Gainesville, FL, 32608, USA.
6
Proteomics and Mass Spectrometry, Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL, 32610, USA.

Abstract

Foliar stomatal movements are critical for regulating plant water loss and gas exchange. Elevated carbon dioxide (CO2 ) levels are known to induce stomatal closure. However, the current knowledge on CO2 signal transduction in stomatal guard cells is limited. Here we report metabolomic responses of Brassica napus guard cells to elevated CO2 using three hyphenated metabolomics platforms: gas chromatography-mass spectrometry (MS); liquid chromatography (LC)-multiple reaction monitoring-MS; and ultra-high-performance LC-quadrupole time-of-flight-MS. A total of 358 metabolites from guard cells were quantified in a time-course response to elevated CO2 level. Most metabolites increased under elevated CO2 , showing the most significant differences at 10 min. In addition, reactive oxygen species production increased and stomatal aperture decreased with time. Major alterations in flavonoid, organic acid, sugar, fatty acid, phenylpropanoid and amino acid metabolic pathways indicated changes in both primary and specialized metabolic pathways in guard cells. Most interestingly, the jasmonic acid (JA) biosynthesis pathway was significantly altered in the course of elevated CO2 treatment. Together with results obtained from JA biosynthesis and signaling mutants as well as CO2 signaling mutants, we discovered that CO2 -induced stomatal closure is mediated by JA signaling.

KEYWORDS:

Arabidopsis thaliana ; Brassica napus ; carbon dioxide; guard cell; jasmonate signaling; metabolomics; reactive oxygen species; stomatal movement

PMID:
27500669
DOI:
10.1111/tpj.13296
[Indexed for MEDLINE]
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