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Dev Comp Immunol. 2016 Dec;65:299-308. doi: 10.1016/j.dci.2016.08.001. Epub 2016 Aug 3.

Cloning and comparative analysis the proximal promoter activities of arginase and agmatinase genes in Apostichopus japonicus.

Author information

1
School of Marine Sciences, Ningbo University, Ningbo, 315211, PR China.
2
School of Marine Sciences, Ningbo University, Ningbo, 315211, PR China. Electronic address: lichenghua@nbu.edu.cn.
3
Agricultural Center, Louisiana State University, United States.

Abstract

Our previous work demonstrated that Apostichopus japonicus arginase and agmatinase from l-arginine metabolism synergistically compete with NOS under pathogens challenge. Here we conducted a study to further investigate the mechanism in the regulation of arginase and agmatinase genes in l-arginine metabolism using EPC cell system. Luciferase analysis and progressive 5' deletion analysis suggested that Ajagmatinase promoter was a very robust promoter for its transcription, and the core region of Ajarginase promoter was located within -277 bp to -157 bp. Besides, their promoter activities were significantly activated by LPS and l-arginine challenge both in a time- and dose-dependent manners in EPC cells. When different truncated reporter vector and expression vector co-transfection experiment revealed transcription factor NF-κB/Rel and STAT5 could significantly inhibited Ajarginase promoter activity, but not Ajagmatinase. Our findings were provided novel insights into the transcriptional regulation of Ajarginase and Ajagmatinase, and selectively change their expressions might prevent pathogens infection.

KEYWORDS:

Apostichopus japonicus; LPS; Luciferase assay; Promoter activity; Transcription factor; l-arginine

PMID:
27497871
DOI:
10.1016/j.dci.2016.08.001
[Indexed for MEDLINE]

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