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Oncotarget. 2016 Aug 23;7(34):55663-55676. doi: 10.18632/oncotarget.10971.

CD24 promoted cancer cell angiogenesis via Hsp90-mediated STAT3/VEGF signaling pathway in colorectal cancer.

Wang X1,2, Zhang Y3, Zhao Y1,2, Liang Y1,2, Xiang C1,2, Zhou H4, Zhang H5,6, Zhang Q1,2, Qing H1,2, Jiang B1,2, Xiong H6, Peng L1,2,6.

Author information

1
Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
2
Guangdong Provincial Key Laboratory of Gastroenterology, Guangzhou 510515, China.
3
Department of Gastroenterology, The First People's Hospital of Yunnan Province, Kunming 65003, China.
4
Department of Ultrasound Imaging, 306 Hospital of PLA, Beijing 100101, China.
5
Institute of Immunology and Molecular Medicine, Jining Medical College, Jining 272067, China.
6
Department of Medicine, Immunology Institute, Icahn School of Medicine at Mount Sinai, New York 10029, NY, USA.

Abstract

CD24 is involved in tumor progression of various cancers, but the effects of CD24 on tumor angiogenesis in colorectal cancer are still unknown. We aimed to investigate the underlying mechanism and role of CD24 on colorectal cancer (CRC) angiogenesis. Our data showed that the microvessal density (MVD) was related to the expression of CD24 in primary and metastasis CRC. Silencing of CD24 could dramatically decrease human umbilical vein endothelial cell (HUVEC) migration, invasion and tubule formation, but trivially affected cell proliferation. We also mechanically showed that silencing CD24 could downregulate the expression of VEGF via inhibiting the phosphorylation and translocation of STAT3. Moreover, Hsp90 was identified as the down-interaction protein of CD24 with co-immunoprecipitation assay and systematic mass spectrometry. Immunofluorescence results showed Hsp90 partly co-localized with CD24 in CRC cell membrane and there was a positive correlation between CD24 and Hsp90 expression in CRC tissues. We gradually evidenced that Hsp90 modulated the stability and degradation of CD24 in a proteasome-depended manner, and transferred the signal transmission from CD24 to STAT3. 17-AAG, a specific Hsp90, could abrogate the CD24 induce- HUVEC migration, invasion and tubule formation in vitro and in vivo. Collectively, our results suggested that CD24 induced CRC angiogenesis in Hsp90-dependent manner and activated STAT3-mediated transcription of VEGF. We provided a new insight into the regulation mechanism of tumor angiogenesis by exploring the role of CD24 in angiogenesis.

KEYWORDS:

CD24; Hsp90; VEGF; angiogenesis; colorectal cancer

PMID:
27494878
PMCID:
PMC5342444
DOI:
10.18632/oncotarget.10971
[Indexed for MEDLINE]
Free PMC Article

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