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Biosens Bioelectron. 2016 Dec 15;86:885-891. doi: 10.1016/j.bios.2016.07.026. Epub 2016 Jul 9.

A viscosity sensitive fluorescent dye for real-time monitoring of mitochondria transport in neurons.

Author information

1
Department of Chemistry and Nano Science, EwhaWomans University, Seoul, 120-750, Republic of Korea.
2
Department of Energy Systems Research, Ajou University, Suwon, 443-749, Republic of Korea.
3
Research Centre for Chemical Biology, Department of Chemistry, Yanbian University, Yanji, 133002, PR China; Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Ministry of Education, Yanbian University, Yanji, 133002, PR China. Electronic address: zhouxin@ybu.edu.cn.
4
Department of Energy Systems Research, Ajou University, Suwon, 443-749, Republic of Korea. Electronic address: kimhm@ajou.ac.kr.
5
Department of Chemistry and Nano Science, EwhaWomans University, Seoul, 120-750, Republic of Korea. Electronic address: jyoon@ewha.ac.kr.

Abstract

We present here a viscosity sensitive fluorescent dye, namely thiophene dihemicyanine (TDHC), that enables the specific staining of mitochondria. In comparison to the common mitochondria tracker (Mitotracker Deep Red, MTDR), this dye demonstrated its unique ability for robust staining of mitochondria with high photostability and ultrahigh signal-to-noise ratio (SNR). Moreover, TDHC also showed high sensitivity towards mitochondria membrane potential (ΔΨm) and intramitochondria viscosity change. Consequently, this dye was utilized in real-time monitoring of mitochondria transport in primary cortical neurons. Finally, the Two-Photon Microscopy (TPM) imaging ability of TDHC was also demonstrated.

KEYWORDS:

Fluorescent probe; Mitochondria selective probe; Mitochondria transport in neurons; Two photon probe; Viscosity probe

PMID:
27494813
DOI:
10.1016/j.bios.2016.07.026
[Indexed for MEDLINE]

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