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Methods Mol Biol. 2016;1460:337-43. doi: 10.1007/978-1-4939-3810-0_23.

Analysis of Aerobic Respiration in Intact Skeletal Muscle Tissue by Microplate-Based Respirometry.

Author information

1
Department of Molecular Virology, Immunology, and Medical Genetics, The Ohio State University College of Medicine, 460 W. 12th Avenue, Columbus, OH, 43210, USA.
2
Center for Muscle Health and Neuromuscular Disorders, The Ohio State University College of Medicine, 460 W. 12th Avenue, Columbus, OH, 43210, USA.
3
Molecular, Cellular and Developmental Biology Graduate Program, The Ohio State University College of Medicine, 460 W. 12th Avenue, Columbus, OH, 43210, USA.
4
Department of Molecular Virology, Immunology, and Medical Genetics, The Ohio State University College of Medicine, 460 W. 12th Avenue, Columbus, OH, 43210, USA. denis.guttridge@osumc.edu.
5
Center for Muscle Health and Neuromuscular Disorders, The Ohio State University College of Medicine, 460 W. 12th Avenue, Columbus, OH, 43210, USA. denis.guttridge@osumc.edu.

Abstract

Mitochondrial function is a key component of skeletal muscle health, and its dysfunction has been associated with a wide variety of diseases. Microplate-based respirometry measures aerobic respiration of live cells through extracellular changes in oxygen concentration. Here, we describe a methodology to measure aerobic respiration of intact murine skeletal muscle tissue. The tissues are not cultured, permeabilized, or enzymatically dissociated to single fibers, so there is minimal experimental manipulation affecting the samples prior to acquiring measurements.

KEYWORDS:

Aerobic respiration; Mitochondria; Oxygen consumption rate; Seahorse; Skeletal muscle

PMID:
27492183
DOI:
10.1007/978-1-4939-3810-0_23
[Indexed for MEDLINE]

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