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FEBS Lett. 2016 Sep;590(18):3168-78. doi: 10.1002/1873-3468.12341. Epub 2016 Sep 12.

N-glycosylation in the thermoacidophilic archaeon Sulfolobus acidocaldarius involves a short dolichol pyrophosphate carrier.

Author information

1
Department of Biochemistry, Duke University Medical Center, Durham, NC, USA.
2
Department of Life Sciences, Ben Gurion University of the Negev, Beersheva, Israel.
3
Molecular Biology of Archaea, Institute for Biology II-Microbiology, Albert-Ludwigs-University of Freiburg, Germany.
4
Department of Life Sciences, Ben Gurion University of the Negev, Beersheva, Israel. jeichler@bgu.ac.il.

Abstract

N-glycosylation is a post-translational modification that occurs across evolution. In the thermoacidophilic archaea Sulfolobus acidocaldarius, glycoproteins are modified by an N-linked tribranched hexasaccharide reminiscent of the N-glycans assembled in Eukarya. Previously, hexose-bearing dolichol phosphate was detected in a S. acidocaldarius Bligh-Dyer lipid extract. Here, we used a specialized protocol for extracting lipid-linked oligosaccharides to detect a dolichol pyrophosphate bearing the intact hexasaccharide, as well as its biosynthetic intermediates. Furthermore, evidence for N-glycosylation of two S. acidocaldarius proteins by the same hexasaccharide and its derivatives was collected. These findings thus provide novel insight into archaeal N-glycosylation.

KEYWORDS:

N-glycosylation; Sulfolobus acidocaldarius; archaea; dolichol; thermoacidophile

PMID:
27490243
PMCID:
PMC5039095
DOI:
10.1002/1873-3468.12341
[Indexed for MEDLINE]
Free PMC Article

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