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Tumour Biol. 2016 Oct;37(10):13903-13914. Epub 2016 Aug 3.

Cytosolic Ku70 regulates Bax-mediated cell death.

Author information

1
Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI, USA.
2
General Surgery, University of Michigan Medical School, Ann Arbor, MI, USA.
3
Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI, USA. rkwok@umich.edu.
4
Obstetrics and Gynecology, University of Michigan Medical School, 1150 West Medical Center Drive, Ann Arbor, MI, 48109, USA. rkwok@umich.edu.

Abstract

The first known function of Ku70 is as a DNA repair factor in the nucleus. Using neuronal neuroblastoma cells as a model, we have established that cytosolic Ku70 binds to the pro-apoptotic protein Bax in the cytosol and blocks Bax's cell death activity. Ku70-Bax binding is regulated by Ku70 acetylation in that when Ku70 is acetylated Bax dissociates from Ku70, triggering cell death. We propose that Ku70 may act as a survival factor in these cells such that Ku70 depletion triggers Bax-dependent cell death. Here, we addressed two fundamental questions about this model: (1) Does all Bax, which is a cytosolic protein, bind to all cytosolic Ku70? and (2) Is Ku70 a survival factor in cells types other than neuronal neuroblastoma cells? We show here that, in neuronal neuroblastoma cells, only a small fraction of Ku70 binds to a small fraction of Bax; most Bax is monomeric. Interestingly, there is no free or monomeric Ku70 in the cytosol; most cytosolic Ku70 is in complex with other factors forming several high molecular weight complexes. A fraction of cytosolic Ku70 also binds to cytosolic Ku80, Ku70's binding partner in the nucleus. Ku70 may not be a survival factor in some cell types (Ku70-depletion less sensitive) because Ku70 depletion does not affect survival of these cells. These results indicate that, in addition to Ku70 acetylation, other factors may be involved in regulating Ku70-Bax binding in the Ku70-depletion less sensitive cells because Ku70 acetylation in these cells is not sufficient to dissociate Bax from Ku70 or to activate Bax.

KEYWORDS:

apoptosis; histone deacetylase

PMID:
27488115
PMCID:
PMC5097087
DOI:
10.1007/s13277-016-5202-z
[Indexed for MEDLINE]
Free PMC Article

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