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Sci Rep. 2016 Aug 3;6:30625. doi: 10.1038/srep30625.

A simultaneous diagnosis and genotyping method for global surveillance of cetacean morbillivirus.

Author information

1
National Chiayi University, Chiayi, Taiwan.
2
University of Las Palmas de Gran Canaria, Las Palmas de Gran Canaria, Canary Islands, Spain.
3
University of São Paulo, São Paulo, Brazil.
4
Instituto Baleia Jubarte (Humpback Whale Institute), Caravelas, Brazil.
5
Hawaii Pacific University, Kaneohe, Hawaii, USA.

Abstract

Cetacean morbillivirus (CeMV) is considered one of the most important viral pathogens in cetaceans. CeMV outbreaks of lethal disease have repeatedly been observed in Europe, the Americas, and Australia, while large herds of gregarious species were found to be the likely reservoirs and sources of CeMV infection to susceptible species in the Atlantic and Pacific Oceans. Furthermore, three new strains were detected recently in Hawaii, Brazil and Australia. To clarify the real global distribution of CeMV and possible carriers, we showed a novel technique successfully diagnosing and distinguishing different virus strains (DMV, PWMV and novel CeMVs) using FFPE samples from 1996 to 2011. This efficient method that combines qRT-PCR and high resolution melting (HRM) could be applied to the future retrospective global studies for better understanding of different prevalence and outbreak conditions among ocean basins and the mechanism of variable host response to pathogens.

PMID:
27484954
PMCID:
PMC4971491
DOI:
10.1038/srep30625
[Indexed for MEDLINE]
Free PMC Article

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