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Virol Rep. 2016 Dec;6:53-60.

Modulation of LINE-1 Retrotransposition by a Human SAMHD1 Polymorphism.

Author information

1
Department of Microbiology and Immunology, Albert Einstein College of Medicine Bronx, NY 10461.
2
Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea.
3
Department of Molecular, Cellular, and Developmental Biology, University of Colorado Boulder, Boulder, Colorado, 80309, USA.
4
Department of Pediatrics, Emory University, Atlanta, GA 30322.

Abstract

The HIV-1 restriction factor SAMHD1 has the ability to negatively modulate retrotransposition of the long interspersed element 1(LINE-1). By exploring the ability of human SAMHD1 polymorphisms to inhibit LINE-1, we found that the single nucleotide polymorphism S33A present in the Korean population lose the ability to inhibit LINE-1 retrotransposition. Because SAMHD1 residue S33 is phosphorylated in human cycling and non-cycling cells, we demonstrated that SAMHD1 requires to be either phosphorylated on position 33 or to contain a bulky residue in order to inhibit LINE-1 retrotransposition. Therefore this unique mutation uncouples functions in this important restriction factor.

KEYWORDS:

HIV-1; LINE-1; SAMHD1; SNPs; restrcition; retrotransposition

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