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Rapid Commun Mass Spectrom. 2016 Oct 15;30(19):2164-70. doi: 10.1002/rcm.7694.

The selected matrix influences the matrix-assisted laser desorption/ionization time-of-flight mass spectral patterns of partially deuterated glycosaminoglycan disaccharides.

Author information

1
University of Leipzig, Medical Faculty, Institute of Medical Physics and Biophysics, Härtelstraße 16-18, D-04107, Leipzig, Germany. ariane.nimptsch@medizin.uni-leipzig.de.
2
University of Leipzig, Medical Faculty, Institute of Medical Physics and Biophysics, Härtelstraße 16-18, D-04107, Leipzig, Germany.

Abstract

RATIONALE:

If carbohydrates are investigated by nuclear magnetic resonance (NMR) spectroscopy, they are normally dissolved in deuterated solvents, such as D2 O. The incorporation of deuterium leads to a high complexity of subsequently recorded mass spectra and reduced sensitivity because different deuterated ions become detectable. Here, we demonstrate that the applied matrix-assisted laser desorption/ionization (MALDI) matrix solution has a considerable impact on the observed isotopic distribution.

METHODS:

Unsaturated disaccharides of chondroitin (CS) and dermatan sulfate (DS) were prepared by enzymatic digestion of the polysaccharides in D2 O and analyzed by MALDI time-of-flight mass spectrometry (TOF MS) using 2,5-dihydroxybenzoic acid (DHB) and 9-aminoacridine (9-AA) according to previously published protocols.

RESULTS:

The extent of deuteration of a given compound can be easily determined by using the mass shift between the non-deuterated and deuterated ions. However, such a determination is more difficult when considering sugars due to their high content of exchangeable groups. Therefore, both the solvent and the matrix have a considerable impact on the MS patterns. Additionally, there are significant differences if the spectra are recorded at different laser fluences.

CONCLUSIONS:

Great caution should be taken when the deuterium content of disaccharides is determined by MALDI MS. Aside from the use of non-protic solvents, DHB is the matrix of choice, whereas 9-AA shows a considerable influence on the observed isotope pattern in dependence on the applied laser fluence. Copyright © 2016 John Wiley & Sons, Ltd.

PMID:
27479785
DOI:
10.1002/rcm.7694
[Indexed for MEDLINE]

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