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Biomed Res Int. 2016;2016:8091353. doi: 10.1155/2016/8091353. Epub 2016 Jul 11.

The HPV16 E7 Oncoprotein Disrupts Dendritic Cell Function and Induces the Systemic Expansion of CD11b(+)Gr1(+) Cells in a Transgenic Mouse Model.

Author information

1
Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), 07360 Mexico City, CDMX, Mexico.
2
Unit of Health Sciences, Faculty of Odontology, Universidad Autónoma de Baja California, 21100 Mexicali, BC, Mexico.
3
Research Unit, Hospital Juárez de México, 07760 Mexico City, CDMX, Mexico.
4
McArdle Laboratory for Cancer Research, University of Wisconsin, School of Medicine and Public Health, Madison, WI 53726, USA.

Abstract

OBJECTIVE:

The aim of this study was to analyze the effects of the HPV16 E7 oncoprotein on dendritic cells (DCs) and CD11b(+)Gr1(+) cells using the K14E7 transgenic mouse model.

MATERIALS AND METHODS:

The morphology of DCs was analyzed in male mouse skin on epidermal sheets using immunofluorescence and confocal microscopy. Flow cytometry was used to determine the percentages of DCs and CD11b(+)Gr1(+) cells in different tissues and to evaluate the migration of DCs.

RESULTS:

In the K14E7 mouse model, the morphology of Langerhans cells and the migratory activity of dendritic cells were abnormal. An increase in CD11b(+)Gr1(+) cells was observed in the blood and skin of K14E7 mice, and molecules related to CD11b(+)Gr1(+) chemoattraction (MCP1 and S100A9) were upregulated.

CONCLUSIONS:

These data suggest that the HPV16 E7 oncoprotein impairs the function and morphology of DCs and induces the systemic accumulation of CD11b(+)Gr1(+) cells.

PMID:
27478837
PMCID:
PMC4958469
DOI:
10.1155/2016/8091353
[Indexed for MEDLINE]
Free PMC Article

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