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J Mass Spectrom. 2016 Nov;51(11):1030-1035. doi: 10.1002/jms.3827.

A one-step matrix application method for MALDI mass spectrometry imaging of bacterial colony biofilms.

Author information

1
Department of Chemistry and Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
2
Department of Chemical and Biomolecular Engineering and Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
3
Department of Chemistry and Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA. jsweedle@illinois.edu.

Abstract

Matrix-assisted laser desorption/ionization imaging of biofilms cultured on agar plates is challenging because of problems related to matrix deposition onto agar. We describe a one-step, spray-based application of a 2,5-dihydroxybenzoic acid solution for direct matrix-assisted laser desorption/ionization imaging of hydrated Bacillus subtilis biofilms on agar. Using both an optimized airbrush and a home-built automatic sprayer, region-specific distributions of signaling metabolites and cannibalistic factors were visualized from B. subtilis cells cultivated on biofilm-promoting medium. The approach provides a homogeneous, relatively dry coating on hydrated samples, improving spot to spot signal repeatability compared with sieved matrix application, and is easily adapted for imaging a range of agar-based biofilms.

KEYWORDS:

MALDI MSI; airbrush; automatic sprayer; biofilms; matrix application

PMID:
27476992
PMCID:
PMC5297451
DOI:
10.1002/jms.3827
[Indexed for MEDLINE]
Free PMC Article

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