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PLoS One. 2016 Jul 29;11(7):e0159829. doi: 10.1371/journal.pone.0159829. eCollection 2016.

Novel Method for Differentiating Histological Types of Gastric Adenocarcinoma by Using Confocal Raman Microspectroscopy.

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Division of General Surgery, Department of Surgery, Tungs' Taichung MetroHarbor Hospital, Taichung City, Taiwan.
Department of Applied Chemistry, National Chiayi University, Chia-Yi, Taiwan.
Center of Nano Bio-detection, National Chung Cheng University, Chia-Yi, Taiwan.
Department of Applied Cosmetology, HungKuang University, No.34, Zhongqi Rd., Shalu Township, Taichung County, Taiwan.
Division of Gastroenterology, Department of Internal Medicine, Tungs' Taichung MetroHarbor Hospital, Taichung City, Taiwan.
Department of Pathology, Tungs' Taichung MetroHarbor Hospital, Taichung City, Taiwan.


Gastric adenocarcinoma, a single heterogeneous disease with multiple epidemiological and histopathological characteristics, accounts for approximately 10% of cancers worldwide. It is categorized into four histological types: papillary adenocarcinoma (PAC), tubular adenocarcinoma (TAC), mucinous adenocarcinoma (MAC), and signet ring cell adenocarcinoma (SRC). Effective differentiation of the four types of adenocarcinoma will greatly improve the treatment of gastric adenocarcinoma to increase its five-year survival rate. We reported here the differentiation of the four histological types of gastric adenocarcinoma from the molecularly structural viewpoint of confocal Raman microspectroscopy. In total, 79 patients underwent laparoscopic or open radical gastrectomy during 2008-2011: 21 for signet ring cell carcinoma, 21 for tubular adenocarcinoma, 14 for papillary adenocarcinoma, 6 for mucinous carcinoma, and 17 for normal gastric mucosas obtained from patients underwent operation for other benign lesions. Clinical data were retrospectively reviewed from medical charts, and Raman data were processed and analyzed by using principal component analysis (PCA) and linear discriminant analysis (LDA). Two-dimensional plots of PCA and LDA clearly demonstrated that the four histological types of gastric adenocarcinoma could be differentiated, and confocal Raman microspectroscopy provides potentially a rapid and effective method for differentiating SRC and MAC from TAC or PAC.

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