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Methods Mol Biol. 2016;1451:259-69. doi: 10.1007/978-1-4939-3771-4_17.

Targeted Electroporation in Embryonic, Larval, and Adult Zebrafish.

Author information

1
Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH-4058, Basel, Switzerland. Ming.Zou@fmi.ch.
2
University of Basel, CH-4003, Basel, Switzerland. Ming.Zou@fmi.ch.
3
Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH-4058, Basel, Switzerland. Rainer.Friedrich@fmi.ch.
4
University of Basel, CH-4003, Basel, Switzerland. Rainer.Friedrich@fmi.ch.
5
Department of Neuroscience, Physiology & Pharmacology, University College London, Gower Street, London, WC1E 6BT, UK. i.bianco@ucl.ac.uk.

Abstract

This chapter describes three fast and straightforward methods to introduce nucleic acids, dyes, and other molecules into small numbers of cells of zebrafish embryos, larvae, and adults using electroporation. These reagents are delivered through a glass micropipette and electrical pulses are given through electrodes to permeabilize cell membranes and promote uptake of the reagent. This technique allows the experimenter to target cells of their choice at a particular time of development and at a particular location in the zebrafish with high precision and facilitates long-term noninvasive measurement of biological activities in vivo. Applications include cell fate mapping, neural circuit mapping, neuronal activity measurement, manipulation of activity, ectopic gene expression, and genetic knockdown experiments.

KEYWORDS:

DNA; Electroporation; Gene expression; Optogenetics; RNA; Zebrafish

PMID:
27464813
DOI:
10.1007/978-1-4939-3771-4_17
[Indexed for MEDLINE]

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