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BMC Cancer. 2016 Jul 26;16:534. doi: 10.1186/s12885-016-2520-x.

Pseudo-HE images derived from CARS/TPEF/SHG multimodal imaging in combination with Raman-spectroscopy as a pathological screening tool.

Author information

1
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller University Jena, Helmholtzweg 4, Jena, Germany. thomas.bocklitz@uni-jena.de.
2
Leibniz-Institute of Photonic Technology, Albert-Einstein-Str. 9, Jena, 07745, Germany. thomas.bocklitz@uni-jena.de.
3
Iraqi Centre for Cancer and Medical Genetics Research, Al-Mustansiriya University, Baghdad, Iraq.
4
Institute of Pathology, University Hospital - Friedrich Schiller University Jena, Ziegelmühlenweg 1, Jena, D-07743, Germany.
5
Leibniz-Institute of Photonic Technology, Albert-Einstein-Str. 9, Jena, 07745, Germany.
6
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller University Jena, Helmholtzweg 4, Jena, Germany.
7
Clinic for Internal Medicine IV, Jena University Hospital, Jena, 07747, Germany.
8
Department of Medicine 1, Friedrich-Alexander-University, Erlangen, 91054, Germany.
9
Erlangen Graduate School in Advanced Optical Technologies (SAOT), Friedrich-Alexander Universität Erlangen-Nürnberg, Erlangen, Germany.
10
Institute for Tumor Biology and Experimental Therapy, Georg-Speyer-Haus, Paul-Ehrlich-Straße 42-44, Frankfurt, 60596, Germany.
11
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller University Jena, Helmholtzweg 4, Jena, Germany. juergen.popp@uni-jena.de.
12
Leibniz-Institute of Photonic Technology, Albert-Einstein-Str. 9, Jena, 07745, Germany. juergen.popp@uni-jena.de.

Abstract

BACKGROUND:

Due to the steadily increasing number of cancer patients worldwide the early diagnosis and treatment of cancer is a major field of research. The diagnosis of cancer is mostly performed by an experienced pathologist via the visual inspection of histo-pathological stained tissue sections. To save valuable time, low quality cryosections are frequently analyzed with diagnostic accuracies that are below those of high quality embedded tissue sections. Thus, alternative means have to be found that enable for fast and accurate diagnosis as the basis of following clinical decision making.

METHODS:

In this contribution we will show that the combination of the three label-free non-linear imaging modalities CARS (coherent anti-Stokes Raman-scattering), TPEF (two-photon excited autofluorescence) and SHG (second harmonic generation) yields information that can be translated into computational hematoxylin and eosin (HE) images by multivariate statistics. Thereby, a computational HE stain is generated resulting in pseudo-HE overview images that allow for identification of suspicious regions. The latter are analyzed further by Raman-spectroscopy retrieving the tissue's molecular fingerprint.

RESULTS:

The results suggest that the combination of non-linear multimodal imaging and Raman-spectroscopy possesses the potential as a precise and fast tool in routine histopathology.

CONCLUSIONS:

As the key advantage, both optical methods are non-invasive enabling for further pathological investigations of the same tissue section, e.g. a direct comparison with the current pathological gold-standard.

KEYWORDS:

Cancer detection; Multimodal imaging; Pseudo HE-images; Raman spectroscopy

PMID:
27460472
PMCID:
PMC4962450
DOI:
10.1186/s12885-016-2520-x
[Indexed for MEDLINE]
Free PMC Article

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