Format

Send to

Choose Destination
J Cell Biol. 2016 Aug 1;214(3):333-45. doi: 10.1083/jcb.201603039. Epub 2016 Jul 25.

mito-QC illuminates mitophagy and mitochondrial architecture in vivo.

Author information

1
Medical Research Council Protein Phosphorylation and Ubiquitylation Unit, University of Dundee, Dundee DD1 9SY, Scotland, UK.
2
Division of Cell Signalling and Immunology, University of Dundee, Dundee DD1 9SY, Scotland, UK Dundee Imaging Facility, School of Life Sciences, University of Dundee, Dundee DD1 9SY, Scotland, UK.
3
Dundee Imaging Facility, School of Life Sciences, University of Dundee, Dundee DD1 9SY, Scotland, UK.
4
Medical Research Council Protein Phosphorylation and Ubiquitylation Unit, University of Dundee, Dundee DD1 9SY, Scotland, UK School of Medicine, University of Dundee, Dundee DD1 9SY, Scotland, UK.
5
Medical Research Council Protein Phosphorylation and Ubiquitylation Unit, University of Dundee, Dundee DD1 9SY, Scotland, UK i.ganley@dundee.ac.uk.

Abstract

Autophagic turnover of mitochondria, termed mitophagy, is proposed to be an essential quality-control (QC) mechanism of pathophysiological relevance in mammals. However, if and how mitophagy proceeds within specific cellular subtypes in vivo remains unclear, largely because of a lack of tractable tools and models. To address this, we have developed "mito-QC," a transgenic mouse with a pH-sensitive fluorescent mitochondrial signal. This allows the assessment of mitophagy and mitochondrial architecture in vivo. Using confocal microscopy, we demonstrate that mito-QC is compatible with classical and contemporary techniques in histochemistry and allows unambiguous in vivo detection of mitophagy and mitochondrial morphology at single-cell resolution within multiple organ systems. Strikingly, our model uncovers highly enriched and differential zones of mitophagy in the developing heart and within specific cells of the adult kidney. mito-QC is an experimentally advantageous tool of broad relevance to cell biology researchers within both discovery-based and translational research communities.

PMID:
27458135
PMCID:
PMC4970326
DOI:
10.1083/jcb.201603039
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center