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Mol Cell Biol. 2016 Sep 26;36(20):2543-52. doi: 10.1128/MCB.00150-16. Print 2016 Oct 15.

Id3 Orchestrates Germinal Center B Cell Development.

Author information

1
Department of Molecular Biology, University of California, San Diego, La Jolla, California, USA.
2
Department of Molecular Biology and Bioinformatics and Department of Medicine, University of California, San Diego, La Jolla, California, USA.
3
Department of Molecular Biology, University of California, San Diego, La Jolla, California, USA murre@biomail.ucsd.edu.

Abstract

Previous studies have demonstrated that E proteins induce activation-induced deaminase (AID) expression in activated B cells. Here, we examined the role of Id3 in germinal center (GC) cells. We found that Id3 expression is high in follicular B lineage cells but declines in GC cells. Immunized mice with Id3 expression depleted displayed a block in germinal center B cell maturation, showed reduced numbers of marginal zone B cells and class-switched cells, and were associated with decreased antibody titers and lower numbers of plasma cells. In vitro, Id3-depleted B cells displayed a defect in class switch recombination. Whereas AID levels were not altered in Id3-depleted activated B cells, the expression of a subset of genes encoding signaling components of antigen receptor-, cytokine receptor-, and chemokine receptor-mediated signaling was significantly impaired. We propose that during the GC reaction, Id3 levels decline to activate the expression of genes encoding signaling components that mediate B cell receptor- and or cytokine receptor-mediated signaling to promote the differentiation of GC B cells.

PMID:
27457619
PMCID:
PMC5038142
DOI:
10.1128/MCB.00150-16
[Indexed for MEDLINE]
Free PMC Article

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