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Org Lett. 2016 Aug 5;18(15):3666-9. doi: 10.1021/acs.orglett.6b01717. Epub 2016 Jul 26.

Photoswitchable Spiropyran Dyads for Biological Imaging.

Author information

1
Department of Chemistry, Chemistry Research Laboratory, University of Oxford , Oxford OX1 3TA, U.K.
2
MRC Human Immunology Unit and Wolfson Imaging Centre Oxford, Weatherall Institute of Molecular Medicine, University of Oxford , Oxford OX3 9DS, U.K.

Abstract

The synthesis of a small-molecule dyad consisting of a far-red-emitting silicon rhodamine dye that is covalently linked to a photochromic spironaphthothiopyran unit, which serves as a photoswitchable quencher, is reported. This system can be switched reversibly between the fluorescent and nonfluorescent states using visible light at wavelengths of 405 and 630 nm, respectively, and it works effectively in aqueous solution. Live-cell imaging demonstrates that this dyad has several desirable features, including excellent membrane permeability, fast and reversible modulation of fluorescence by visible light, and good contrast between the bright and dark states.

PMID:
27456166
PMCID:
PMC5010358
DOI:
10.1021/acs.orglett.6b01717
[Indexed for MEDLINE]
Free PMC Article

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