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Eur J Pharmacol. 2016 Oct 15;789:195-201. doi: 10.1016/j.ejphar.2016.07.039. Epub 2016 Jul 22.

Ameliorating effects of 1,8-cineole on LPS-induced human umbilical vein endothelial cell injury by suppressing NF-κB signaling in vitro.

Author information

1
The Key Lab of Optimal Utilization of Natural Medicine Resources, Guizhou Medical University, University Town, Guian New District, Guizhou 550025, China.
2
The Key Lab of Optimal Utilization of Natural Medicine Resources, Guizhou Medical University, University Town, Guian New District, Guizhou 550025, China; Department of Pharmacology of Materia Medica, Guizhou Medical University, University Town, Guian New District, Guizhou 550025, China.
3
The Key Lab of Optimal Utilization of Natural Medicine Resources, Guizhou Medical University, University Town, Guian New District, Guizhou 550025, China; Department of Pharmacology of Materia Medica, Guizhou Medical University, University Town, Guian New District, Guizhou 550025, China. Electronic address: shenxiangchun@126.com.

Abstract

1,8-Cineole (also known as eucalyptol) is a monoterpene that occurs naturally in many aromatic plants, 1,8-cineole has been reported to ameliorate dysfunction of endothelial cells. However, the mechanism of action of 1,8-cineole is incompletely understood. We investigated the protective effect of 1,8-cineole on lipopolysaccharide (LPS)-induced human umbilical vein endothelial cell (HUVEC) injury and the underlying mechanisms. HUVECs were preincubated with 1,8-cineole for 1.5h, then exposed to LPS for 12h. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase leakage assays showed 1,8-cineole reduced LPS-induced HUVEC injury significantly. Results from enzyme linked immunosorbent assays revealed that 1,8-cineole suppressed LPS-induced secretion of interleukin-6 and interleukin-8, and recovered nitric oxide to normal levels. 1,8-Cineole decreased phosphorylation of nuclear factor-kappa B (NF-κB) p65 and expression of inducible nitric oxide synthase, and simultaneously improved protein levels of endothelial nitric oxide synthase. Immunofluorescence confirmed 1,8-cineole moderates nuclear translocation of NF-κB. These results suggest that 1,8-cineole ameliorates HUVEC dysfunction significantly, and that this effect at least involves NF-κB suppression.

KEYWORDS:

1,8-Cineole; Human umbilical vein endothelial cell (HUVEC); Lipopolysaccharide (LPS); Nitric oxide (NO); Nuclear transcription factor-kappa B (NF-κB)

PMID:
27455900
DOI:
10.1016/j.ejphar.2016.07.039
[Indexed for MEDLINE]

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