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Sci Adv. 2016 Jul 15;2(7):e1600500. doi: 10.1126/sciadv.1600500. eCollection 2016 Jul.

Heterogeneity of cellular circadian clocks in intact plants and its correction under light-dark cycles.

Author information

1
Department of Botany, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan.

Abstract

Recent advances in single-cell analysis have revealed the stochasticity and nongenetic heterogeneity inherent to cellular processes. However, our knowledge of the actual cellular behaviors in a living multicellular organism is still limited. By using a single-cell bioluminescence imaging technique on duckweed, Lemna gibba, we demonstrate that, under constant conditions, cells in the intact plant work as individual circadian clocks that oscillate with their own frequencies and respond independently to external stimuli. Quantitative analysis uncovered the heterogeneity and instability of cellular clocks and partial synchronization between neighboring cells. Furthermore, we found that cellular clocks in the plant body under light-dark cycles showed a centrifugal phase pattern in which the effect of cell-to-cell heterogeneity in period lengths was almost masked. The inherent heterogeneity in the properties of cellular clocks observed under constant conditions is corrected under light-dark cycles to coordinate the daily rhythms of the plant body. These findings provide a novel perspective of spatiotemporal architectures in the plant circadian system.

KEYWORDS:

bioluminescence; circadian rhythms; pattern formation; single cell analysis

PMID:
27453946
PMCID:
PMC4956400
DOI:
10.1126/sciadv.1600500
[Indexed for MEDLINE]
Free PMC Article

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