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Reprod Toxicol. 2016 Oct;65:139-147. doi: 10.1016/j.reprotox.2016.05.015. Epub 2016 Jul 21.

Optimizing multi-dimensional high throughput screening using zebrafish.

Author information

1
Department of Environmental and Molecular Toxicology, The Sinnhuber Aquatic Research Laboratory, and the Environmental Health Sciences Center at Oregon State University, Corvallis, OR, USA.
2
Department of Biology, Baylor University, Waco, TX, USA.
3
Department of Environmental and Molecular Toxicology, The Sinnhuber Aquatic Research Laboratory, and the Environmental Health Sciences Center at Oregon State University, Corvallis, OR, USA. Electronic address: Robert.Tanguay@oregonstate.edu.

Abstract

The use of zebrafish for high throughput screening (HTS) for chemical bioactivity assessments is becoming routine in the fields of drug discovery and toxicology. Here we report current recommendations from our experiences in zebrafish HTS. We compared the effects of different high throughput chemical delivery methods on nominal water concentration, chemical sorption to multi-well polystyrene plates, transcription responses, and resulting whole animal responses. We demonstrate that digital dispensing consistently yields higher data quality and reproducibility compared to standard plastic tip-based liquid handling. Additionally, we illustrate the challenges in using this sensitive model for chemical assessment when test chemicals have trace impurities. Adaptation of these better practices for zebrafish HTS should increase reproducibility across laboratories.

KEYWORDS:

Developmental toxicity; High throughput screening; Zebrafish

PMID:
27453428
PMCID:
PMC5067206
DOI:
10.1016/j.reprotox.2016.05.015
[Indexed for MEDLINE]
Free PMC Article

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