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Cell Mol Biol (Noisy-le-grand). 2016 Jun 30;62(7):27-30.

Toxicity of Hoechst 33342: implication in side population analysis.

Author information

1
Children's Hospital of Fudan University Department of Surgery Shanghai China.
2
Anhui Proveince Children's Hospital Department of Surgery Anhui China.
3
Children's Hospital of Fudan University Department of Surgery Shanghai China xmxiao@shmu.edu.cn.

Abstract

In the current study, we discovered that Hoechst 33342 (H342) could produce significant toxicity to the human neuroblastoma SK-N-SH cells at concentrations typically used to identify side population (SP) cells. At 3.75 and 5 µg/ml, H342 induced cell death and apoptosis. Flow cytometry analysis revealed cell cycle arrest at S-phase. Our findings caution against using H342 for SP analysis without considering differential susceptibility of different cells to H342. Specifically, concentration of H342 needs to be adjusted carefully to minimize toxicity for each cell line.

PMID:
27453268
[Indexed for MEDLINE]

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